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Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor-like subunit with homology to the Drosophila melanogaster Dβ1 subunit

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Invertebrate Neuroscience

Abstract

The cloning, sequencing and functional expression of Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor (nAChR) subunit is described. This subunit shows 80% identity with the Drosophila melanogaster Dβ1 and 92% identity with the Locusta migratoria β1, non-α subunits but only 38% identity to Sgα1 (also referred to as αL1), a previously cloned S. gregaria nAChR α-subunit. When expressed in Xenopus laevis oocytes, Sgβ1 does not respond to nicotine. Responses to nicotine are observed, however, in oocytes co-expressing Sgα1 and Sgβ1, but the pharmacology is indistinguishable from that of currents produced by expressing Sgα1 alone. We conclude that either Sgβ1 does not co-assemble with Sgα1, or that it is unable to contribute to the functional properties of the receptor, in the Xenopus oocyte expression system.

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Acknowledgements

The authors are indebted to Eric Barnard, Ann Stephenson, Leslie Blair, Vince Dionne, Jonathan David, Ed Levitan, Ryuzo Shingai, Anthony Kerlevagge, and Mike Goosey for helpful discussions during the course of this work. The financial support of the Medical Research Council, the Agricultural and Food Research Council and Shell Research Ltd., UK is gratefully acknowledged.

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Authors and Affiliations

  1. MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1, 3QX, UK

    A. K. Jones, S. D. Buckingham & D. B. Sattelle

  2. Brown University, Providence, Box G-B4, Rhode Island, RI 02912-1953, USA

    J. Marshall

  3. Seton Hall University, South Orange, New Jersey, 072079-2694, USA

    A. D. Blake

  4. Department of Life Sciences, Neuroscience and Signal Transduction Laboratory, School of Biomedical and Natural Sciences, College of Science and Technology, Nottingham Trent University, Clifton Campus, Clifton Lane, Nottingham, NG11 8NS, UK

    M. G. Darlison

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  1. A. K. Jones
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  2. J. Marshall
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  5. M. G. Darlison
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  6. D. B. Sattelle
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Correspondence to D. B. Sattelle.

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Jones, A.K., Marshall, J., Blake, A.D. et al. Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor-like subunit with homology to the Drosophila melanogaster Dβ1 subunit. Invert Neurosci 5, 147–155 (2005). https://doi.org/10.1007/s10158-005-0007-6

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  • DOI: https://doi.org/10.1007/s10158-005-0007-6

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