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Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis)

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Abstract

To develop a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis), 148 microsatellite markers were selected from the human genome database. The polymorphisms and inheritance of PCR products were determined by screening twenty unrelated monkeys and by analysis of three families, respectively. As a result, 106 primers (72%) gave PCR products of the size expected for humans and rhesus monkeys. Among these products, polymorphism and single-gene inheritance in cynomolgus monkeys was observed for 66 markers (62%). The average number of alleles at the 66 polymorphic loci was 5.86 (range 2–10), and average heterozygosity was 0.63 (range 0.10–0.88). This is the first report of microsatellite markers for cynomolgus monkeys. Chromosomal mapping of these markers is now in progress.

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Acknowledgments

We are grateful to Dr Duncan A. Vaughan (The National Institute of Agrobiological Sciences, Japan) for critical reading and editing of the manuscript. This study was supported by a grant (H15-genome-004) from the Ministry of Health, Labor, and Welfare, Japan.

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Authors and Affiliations

  1. Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, Tskukuba, Ibaraki, 305-0843, Japan

    Toshihiko Kikuchi, Masayuki Hara & Keiji Terao

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  1. Toshihiko Kikuchi
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  2. Masayuki Hara
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  3. Keiji Terao
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Correspondence to Keiji Terao.

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Kikuchi, T., Hara, M. & Terao, K. Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis). Primates 48, 140–146 (2007). https://doi.org/10.1007/s10329-006-0008-z

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  • DOI: https://doi.org/10.1007/s10329-006-0008-z

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