Abstract
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 μg AHL h−1 per 109 CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif 106HXDH-59 amino acids-H169-21 amino acids-D191 for N-acylhomoserine lactone lactonases.
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Acknowledgements
We gratefully acknowledge the support of three grants from the University of Malaya, namely, Vot F Research Grant (FS260/2008C), Research University Grants (TA048-2008A, RG003/09BIO), both to KG Chan, and Postgraduate Research Grant (PS256/2008A) to CS Wong.
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Chan, KG., Wong, CS., Yin, WF. et al. Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil. Antonie van Leeuwenhoek 98, 299–305 (2010). https://doi.org/10.1007/s10482-010-9438-0
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DOI: https://doi.org/10.1007/s10482-010-9438-0