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A rapid detection method for apoptosis and necrosis measurement using the Cellometer imaging cytometry

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Abstract

Apoptosis and necrosis play an important role in various aspects of preclinical pharmaceutical drug discovery and validation. The ability to quickly determine the cytotoxic effect of chemical compounds on cancer cells allows researchers to efficiently identify potential drug candidates for further development in the pharmaceutical discovery pipeline. Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis. Currently, fluorescence-based cell death assays have not been demonstrated by the Cellometer system, which can potentially provide a quick, simple, and inexpensive alternative method for smaller biomedical research laboratories. In this study, we demonstrate for the first time the use of Cellometer imaging cytometry for necrosis/apoptosis detection by studying the dose–response effect of heat and drug-induced cell death in Jurkat cells labeled with annexin V-FITC (apoptotic) and propidium iodide (necrotic). The experimental results were evaluated to validate the imaging cytometric capabilities of the Cellometer system as compared to the conventional flow cytometry. Similar cell population results were obtained from the two methods. The ability of Cellometer to rapidly and cost-effectively perform fluorescent cell-based assays has the potential of improving research efficiency, especially where a flow or laser scanning cytometer is not available or in situations where a rapid analysis of data is desired.

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Conflict of interest

The authors, LLC, NL, TS, and BL declare competing financial interests, and the work performed in this manuscript is for reporting on instrument performance of Nexcelom Bioscience, LLC. The performance of the system has been compared to standard approaches currently used in the biomedical research institutions.

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Authors and Affiliations

  1. Department of Technology R&D, Nexcelom Bioscience LLC, 360 Merrimack St. Building 9, Lawrence, MA, 01843, USA

    Leo Li-Ying Chan

  2. Department of Applications, Nexcelom Bioscience LLC, Lawrence, MA, 01843, USA

    Ning Lai, Tim Smith & Bo Lin

  3. Department of Cellular Analysis, BioLegend®, 11080 Roselle Street, San Diego, CA, 92121, USA

    Elena Wang & Xifeng Yang

Authors
  1. Leo Li-Ying Chan
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  2. Ning Lai
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  3. Elena Wang
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  4. Tim Smith
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  5. Xifeng Yang
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  6. Bo Lin
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Correspondence to Leo Li-Ying Chan.

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Chan, L.LY., Lai, N., Wang, E. et al. A rapid detection method for apoptosis and necrosis measurement using the Cellometer imaging cytometry. Apoptosis 16, 1295–1303 (2011). https://doi.org/10.1007/s10495-011-0651-8

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  • DOI: https://doi.org/10.1007/s10495-011-0651-8

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