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Construction of the bifunctional enzyme cellulase-β-glucosidase from the hyperthermophilic bacterium Thermotoga maritima

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Abstract

An artificial bifunctional enzyme, cellulase-β-glucosidase, was prepared by gene fusion from the hyperthermophilic bacterium Thermotoga maritima MSB8. The fusion protein exhibited both cellulase (Cel5C) and β-glucosidase (BglB) activity when the bglB gene was fused to downstream of cel5C, but not when cel5C was fused to downstream of bglB. The specific activity of the bifunctional enzyme was 70% lower than that of cellulase or β-glucosidase. The fusion enzyme was purified, and the MW was estimated as 114 kDa. The fusion enzyme displayed optimum cellulase activity at pH 8.0 and 70°C over 30 min, and optimal β-glucosidase activity at pH 7.0 and 80°C over 30 min.

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Acknowledgments

This work was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology, and ARPC Program, Ministry of Agriculture and Forestry, Republic of Korea. S.Y. Hong is supported by scholarships from the BK21 program, Ministry of Education & Human Resources Development, Korea.

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Authors and Affiliations

  1. Division of Applied Life Science, Gyeongsang National University, Chinju, 660-701, Korea

    Su-Young Hong, Jin-Suk Lee, Kye-Man Cho, Renukaradhya K. Math, Yong-Hee Kim, Sun-Joo Hong & Han-Dae Yun

  2. Research Institute of Agriculture & Life Science, Gyeongsang National University, Chinju, 660-701, Korea

    Han-Dae Yun

  3. Department of Microbiological Engineering, Chinju National University, Chinju, 660-758, Korea

    Yong-Un Cho & Soo-Jeong Cho

  4. Department of Agricultural Chemistry, Sunchon National University, Sunchon, 540-742, Korea

    Hoon Kim

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  1. Su-Young Hong
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  2. Jin-Suk Lee
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Correspondence to Han-Dae Yun.

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Hong, SY., Lee, JS., Cho, KM. et al. Construction of the bifunctional enzyme cellulase-β-glucosidase from the hyperthermophilic bacterium Thermotoga maritima . Biotechnol Lett 29, 931–936 (2007). https://doi.org/10.1007/s10529-007-9334-5

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  • DOI: https://doi.org/10.1007/s10529-007-9334-5

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