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Microbial consumption and production of volatile organic compounds at the soil-litter interface

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Abstract

Substantial amounts of volatile organic compounds (VOCs) can be released during decomposition and these compounds can affect atmospheric chemistry, belowground processes, and the structure of microbial communities in litter and soil. However, we have a limited understanding of the types, quantities and ecological impacts of VOCs emitted from litter. Here we used a closed flow-through system and proton transfer reaction mass spectrometry (PTR-MS) to characterize VOC emissions from soil and two litter types (Pinus taeda and Acer rubrum) over a 72-day incubation period. Microbial respiration rates were measured throughout the incubation, and the soils were harvested at the end of the incubation to determine how litter VOCs influenced soil C dynamics, N mineralization rates, and bacterial communities. Using the PTR-MS we identified over 100 VOCs, with 10 VOCs making up the majority of emissions. VOCs accounted for up to 2.5% of the C flux from litter. Soil was a net sink of litter VOCs, absorbing up to 80% of VOCs released by litter, and exposure of soil to litter VOCs increased microbial respiration rates in soil by up to 15%. However, we observed negligible impacts of litter VOCs on soil nutrient levels and bacterial community structure, suggesting that soils must be exposed to higher concentrations of VOCs than observed in our study, to cause effects on these soil characteristics. Overall, VOCs appear to have an important influence on C dynamics at the soil-litter interface and VOC emissions from decomposing litter may represent an understudied component of biosphere–atmosphere interactions.

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Acknowledgements

We would like to thank members of the Fierer lab for their valuable comments on previous drafts of this manuscript. We would also like to thank Mark Bradford and Mike Strickland for their help with sample collection, Rob Knight and Micah Hamady for their help with the sequence analyses, Michael Wilkinson for his guidance and aide in sampling, and Russ Monson for making the PTR-MS available for our use. Finally, we thank the three anonymous reviewers for their thoughtful comments. This work was funded by grants awarded to N.F. from the National Science Foundation and the Andrew W. Mellon Foundation.

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Authors and Affiliations

  1. Department of Ecology and Evolutionary Biology, University of Colorado, UCB 334, Boulder, CO, 80309-0334, USA

    Kelly S. Ramirez & Noah Fierer

  2. Cooperative Institute for Research in Environmental Sciences, University of Colorado, UCB 216, Boulder, CO, 80309, USA

    Christian L. Lauber & Noah Fierer

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  1. Kelly S. Ramirez
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  2. Christian L. Lauber
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  3. Noah Fierer
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Correspondence to Kelly S. Ramirez.

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Ramirez, K.S., Lauber, C.L. & Fierer, N. Microbial consumption and production of volatile organic compounds at the soil-litter interface. Biogeochemistry 99, 97–107 (2010). https://doi.org/10.1007/s10533-009-9393-x

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