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Characterization of ferric-anguibactin transport in Vibrio anguillarum

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Abstract

The fish pathogen Vibrio anguillarum is the causative agent of a fatal hemorrhagic septicemia in salmonid fish. Many serotype O1 strains harbors a 65 Kbp plasmid (pJM1 encoding an iron sequestering system essential for virulence. The genes involved in the biosynthesis of the indigenous siderophore anguibactin are encoded by both the pJM1 plasmid and the chromosome, while those involved in the transport of the ferric-siderophore complex, including the outer membrane receptor, are plasmid-encoded. This work describes the role of specific amino acid residues of the outer membrane receptor FatA in the mechanism of transport of ferric-anguibactin. FatA modeling indicated that this protein has a 22 stranded ß-barrel blocked by the plug domain, the latter being formed by residues 51–54. Deletion of the plug domain resulted in a receptor unable to act as an open channel for the transport of the ferric anguibactin complex.

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Acknowledgments

This work was supported by grants AI19018 and GM64600 from the National Institutes of Health to JHC.

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  1. Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, 97239, USA

    Claudia S. López & Jorge H. Crosa

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  1. Claudia S. López
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  2. Jorge H. Crosa
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Correspondence to Jorge H. Crosa.

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López, C.S., Crosa, J.H. Characterization of ferric-anguibactin transport in Vibrio anguillarum . Biometals 20, 393–403 (2007). https://doi.org/10.1007/s10534-007-9084-9

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