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Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass

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Abstract

We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms and cells in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms and cells.

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Authors and Affiliations

  1. RIKEN-The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako, Saitama, 351–0198, Japan

    Yasutaka Hanada, Koji Sugioka & Katsumi Midorikawa

  2. RIKEN-Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Hiroyuki Kawano, Ikuko Shihira Ishikawa & Atsushi Miyawaki

Authors
  1. Yasutaka Hanada
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  2. Koji Sugioka
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  3. Hiroyuki Kawano
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  4. Ikuko Shihira Ishikawa
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  5. Atsushi Miyawaki
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  6. Katsumi Midorikawa
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Correspondence to Yasutaka Hanada.

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Hanada, Y., Sugioka, K., Kawano, H. et al. Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass. Biomed Microdevices 10, 403–410 (2008). https://doi.org/10.1007/s10544-007-9149-0

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  • DOI: https://doi.org/10.1007/s10544-007-9149-0

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