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Proteomic profiling of recombinant cells from large-scale mammalian cell culture processes

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Abstract

Global expression profiling of mammalian cells used for the production of biopharmaceuticals will allow greater insights into the molecular mechanisms that result in a high producing cellular phenotype. These studies may give insights for genetic intervention to possibly create better host cell lines or even to provide clues to more rational strategies for cell line and process development. In this review I will focus on the contribution of proteomic technologies to a greater understanding of the biology of Chinese hamster ovary cells and other producing cell lines such as NS0 mouse cells.

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Abbreviations

CHO:

Chinese hamster ovary

MS:

mass spectrometry

PAGE:

Polyacrylamide Gel Electrophoresis

Mab:

monoclonal antibody

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Acknowledgements

This work is funded by Science Foundation Ireland (SFI).

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  1. National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland

    Paula Meleady

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  1. Paula Meleady
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Correspondence to Paula Meleady.

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Meleady, P. Proteomic profiling of recombinant cells from large-scale mammalian cell culture processes. Cytotechnology 53, 23–31 (2007). https://doi.org/10.1007/s10616-007-9052-9

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  • DOI: https://doi.org/10.1007/s10616-007-9052-9

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