Skip to main content

Advertisement

Log in

Expression of NF-κB in Helicobacter pylori Infection

  • Original Article
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Helicobacter pylori colonizes the gastric mucosa in humans and causes chronic gastritis. NF-κB has a key role as a mediator in mucosal inflammation. In this study, we examined the expression of NF-κB in the antral epithelial cells of H. pylori-infected and H. pylori-uninfected biopsies and examined these processes in relationship with grade and activity of gastritis, density of H. pylori, presence of the intestinal metaplasia, and atrophy.

Fifty biopsies (35 H. pylori-positive patients and 15 H. pylori-negative controls) were studied. NF-κB immunohistochemical stain was performed. NF-κB activity in H. pylori-infected biopsies were markedly enhanced compared with uninflamed biopsies (P = 0.001). We also found positive correlation NF-κB expression with severity of gastritis (according to Sydney score) (P = 0.001), activity of gastritis (P = 0.046) and H. pylori load (P < 0.001), and atrophy (P = 0.004). We did not find a significant relationship between NF-κB and the presence of intestinal metaplasia (P = 0.355). These findings suggested that expression of NF-κB has an important role in H. pylori gastritis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Versalovic J (2003) Helicobacter pylori. Am J Clin Pathol 119:403–412

    Article  PubMed  Google Scholar 

  2. Tummala S, Keates S, Kelly CP (2004) Update on immunologic basis of Helicobacter pylori gastritis. Curr Opin Gastroenterol 20:592–597

    Article  CAS  PubMed  Google Scholar 

  3. Kacar F, Çulhaci N, Yükselen V, Meteoglu I (2004) Histologic demonstration of Helicobacter pylori in gastric biopsies: which is the best staining method? Internet J Pathol 3:1

    Google Scholar 

  4. Zambon C (2005) Pro-and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection: interactions influence outcome. Cytokine 29:141–152

    Article  CAS  PubMed  Google Scholar 

  5. Lehmann F (2002) In situ correlation of cytokine secretion and apoptosis in Helicobacter pylori-associated gastritis. AJP-Gastrointest Liver Physiol 283:481–488

    Google Scholar 

  6. Ley C (2004) Helicobacter pylori eradication and gastric preneoplastic conditions: a randomized, double-blind, placebo-controlled trial. Cancer Epidemiol Biomarkers Prev 13:4–10

    Article  CAS  PubMed  Google Scholar 

  7. Suganuma M (2001) Helicobacter pylori membrane protein 1: a new carcinogenic factor of Helicobacter pylori. Cancer Res 61:6356–6359

    CAS  PubMed  Google Scholar 

  8. Lindholm C (1998) Local cytokine response in Helicobacter pylori-infected subjects. Infect Immunol 66:5964–5971

    CAS  Google Scholar 

  9. Normark S (2003) Persistent infection with Helicobacter pylori and the development of gastric cancer. Adv Cancer Res 90:63–89

    Article  PubMed  Google Scholar 

  10. Odenbreit S (2000) Translocation of Helicobacter pylori cagA into gastric epithelial cells by type IV secretion. 287:1497–1500

  11. Quiding-Jarbrink M (2001) CD4 (and CD8 (T cell responses in Helicobacter pylori-infected individuals. Clin Exp Immunol 123:81–87

    Article  CAS  PubMed  Google Scholar 

  12. Bamford KB (1998) Lymphocytes in the human gastric mucosa during Helicobacter pylori have a T helper cell 1 phenotype. Gastroenterology 114:482–492

    Article  CAS  PubMed  Google Scholar 

  13. Young Lee S (1998) Stimulus-dependent synergism of the antiapoptotic tumor necrosis factor receptor-associated factor 2 (TRAF2) and nuclear factor κB pathways. J Exp Med 7:1381–1384

    Google Scholar 

  14. Suganuma M (2005) New tumor necrosis factor- a-inducing protein released from Helicobacter pylori for gastric cancer progression. J Cancer Res Clin Oncol 131:305–313

    Article  CAS  PubMed  Google Scholar 

  15. Van den Brink G (2000) Expression and activation of NF-κB in the antrum of the human stomach. J Immunol 164:3353–3359

    CAS  PubMed  Google Scholar 

  16. Barnes PJ, Karin M (1997) Nuclear factor-kappa B: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med 336:1066–1071

    Article  CAS  PubMed  Google Scholar 

  17. Sha WC (1998) Regulation of immune responses by NF-κB/Rel transcription factors. J Exp Med 187:143

    Article  CAS  PubMed  Google Scholar 

  18. Baeuerle PA (1998) 1κB- NF-κB structures:at the interface of inflammation control. Cell 95:729

    Article  CAS  PubMed  Google Scholar 

  19. Rogler GK (1998) Nuclear factor κB is activated in macrophages and epithelial cells of inflamed intestinal mucosa. Gastroenterology 115:357

    Article  CAS  PubMed  Google Scholar 

  20. Dixon MF (1996) Classification and grading of gastritis. Am J Surg Pathol 20:1161–1181

    Article  CAS  PubMed  Google Scholar 

  21. Yang GF, Deng CS, Xiong YY, Gong LL, Wang BC, Luo J (2004) Expression of nuclear factor-kappa B and target genes in gastric precancerous lesions and adenocarcinoma: association with Helicobacter pylori cagA (+) infection. World J Gastroenterol 10:491–496

    CAS  PubMed  Google Scholar 

  22. Reuther-Madrid JY, Kashatus D, Chen S, Li X, Westwick J (2002) The p65/RelA subunit of NF-kappa B suppresses the sustained, antiapoptotic activity of Jun kinase induced by tumor necrosis factor. Mol Cell Biol 22:8175–8183.

    Article  CAS  PubMed  Google Scholar 

  23. Sawada M (1997) The G cells. Annu Rev Physiol 59:273

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Furuzan Kacar Doger.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Doger, F.K., Meteoglu, I., Ozkara, E. et al. Expression of NF-κB in Helicobacter pylori Infection. Dig Dis Sci 51, 2306–2309 (2006). https://doi.org/10.1007/s10620-006-9352-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10620-006-9352-5

Keywords

Navigation