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Down-Regulation of miR-27a Might Reverse Multidrug Resistance of Esophageal Squamous Cell Carcinoma

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Abstract

Background

So far, the miRNAs involved in multidrug resistance of esophageal cancer have not been reported.

Aims and Methods

Here we have firstly investigated the roles of miR-27a in multidrug resistance of esophageal squamous cell carcinoma using MTT assay, flow cytometry assay, and reporter gene assay, etc.

Results

Down-regulation of miR-27a could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-non-related drugs on esophageal cancer cells, and might promote ADR-induced apoptosis, accompanied by increased accumulation and decreased releasing amount of ADR. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein, Bcl-2, and the transcription of the multidrug resistance gene 1, but up-regulate the expression of Bax.

Conclusions

MiR-27a might play important roles in multidrug resistance of esophageal cancer. The further study of the biological functions of miR-27a might be helpful for developing possible strategies to treat esophageal cancer.

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Abbreviations

VCR:

Vincristine

ADR:

Adriamycin

5-Flu:

5-Fluorouracil

CDDP:

Cisplatin

FCM:

Flow cytometry

miRNA:

microRNA

MTT:

3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide

MDR:

Multidrug resistance

P-gp:

P-glycoprotein

MRP:

Multidrug resistance-associated protein

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Acknowledgments

This study was supported in part by grants from the National Scientific Foundation of China (30770958 and 30871141).

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Authors and Affiliations

  1. Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 710032, Xi’an, Shaanxi Province, China

    Hongwei Zhang, Mengbin Li, Liu Hong, Taiqian Gong, Li Sun & Xiushan Zheng

  2. Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, Shaanxi Province, China

    Yu Han

Authors
  1. Hongwei Zhang
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  2. Mengbin Li
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  3. Yu Han
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  4. Liu Hong
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  5. Taiqian Gong
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  6. Li Sun
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  7. Xiushan Zheng
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Corresponding authors

Correspondence to Hongwei Zhang or Liu Hong.

Additional information

Hongwei Zhang, Mengbin Li, Yu Han, and Liu Hong contributed equally to this work.

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Zhang, H., Li, M., Han, Y. et al. Down-Regulation of miR-27a Might Reverse Multidrug Resistance of Esophageal Squamous Cell Carcinoma. Dig Dis Sci 55, 2545–2551 (2010). https://doi.org/10.1007/s10620-009-1051-6

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  • DOI: https://doi.org/10.1007/s10620-009-1051-6

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