Abstract
The nematicidal activity of saponins from Medicago arborea (tops), M. arabica (tops and roots) and M. sativa (tops and roots) against the plant-parasitic nematode Xiphinema index was investigated. Nematicidal activity of related prosapogenins and sapogenins on X. index is also described. Saponins from Medicago spp. at different concentrations were all nematicidal, those from M. arborea tops being the less effective. In general, saponins induced 100% mortality at 500 μg ml−1 between 8 and 48 h, while prosapogenins resulted in toxicity starting at 125 μg ml−1. Differences in the effects on X. index induced by prosapogenins and sapogenins were less pronounced, although prosapogenins displayed a larger range of activity. Assays with purified sapogenins demonstrated the relationship of the observed nematicidal activity of M. sativa and M. arborea to the content of the main aglycones (medicagenic acid and hederagenin, respectively) in the saponin extracts. Hederagenin displayed the highest bioactivity, giving 38% mortality after 1 h at 125 μg ml−1.
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Avato, P., Bucci, R., Tava, A., Vitali, C., Rosato, A., Bialy, Z. et al. (2005). Antimicrobial activity of saponins from Medicago sp.: Structure–activity relationship. Phytotherapy Research, 20, 454–457.
Bialy, Z., Jurzysta, M., Mella, M., & Tava, A. (2004). Triterpene saponins from aerial parts of Medicago arabica L. Journal of Agricultural and Food Chemistry, 52, 1095–1099.
Bialy, Z., Jurzysta, M., Mella, M., & Tava, A. (2006). Triterpene saponins from the roots of Medicago hybrida (Pourret) Trautv. Journal of Agriculture and Food Chemistry, 54, 2520–2526.
Chitwood, D. J. (2002). Phytochemical based strategies for nematode control. Annual Reviews of Phytopathology, 40, 221–249.
D’Addabbo, T., Curto, G., Greco, P., Di Silvestro, D., Coiro, M.I., Lamberti, F., et al. (2005). Prove preliminari di Lotta contro nematodi galligeni mediante estratti di Quillaja saponaria Molina. Nematologia Mediterranea 33, 29–34.
Finney, D. J. (1978). Statistical methods in biological assay. London, UK: C. Griffith.
Francis, G., Keem, Z., Makkar, H. P. S., & Becker, K. (2002). The biological action of saponins in animal systems. A review. British Journal of Nutrition, 88, 587–605.
Heng, L., Koningsveld van, G. A., Gruppen, H., Boekel van, M. A. J. S., Vincken, J-P., Roozen, J. P. et al. (2004). Protein–flavour interactions in relation to development of novel protein foods. Trends in Food Science & Technology, 15, 217–224.
Houghton, P., Patel, N., Jurzysta, M., Bialy, Z., & Cheng, C. (2006). Antidermatophyte activity of medicago extracts and contained saponins and their structure–activity relationships. Phytotherapy Research, 20, 1061–1066.
Ikedo, S., Shimoyada, M., & Watanabe, K. (1996). Interaction between bovine serum albumin and saponin as studied by heat stability and protease digestion. Journal of Agricultural and Food Chemistry, 44, 792–795.
Julier, B., Guy, P., Castillo-Acuna, C., Caubel, G., Ecalle, C., Esquibet, M. et al. (1996). Genetic variation for disease and nematode resistances and forage quality in perennial diploid and tetraploid lucerne populations (Medicago sativa L.). Euphytica, 91, 241–250.
Jurzysta, M. (1982). M. Polsky Urzad Patentowy. Patent No. 114171.
Jurzysta, M., Price, K., Ridout, C., & Fenwick, R. (1989). The structure of four triterpenoid saponins isolated from the seed of Trifolium incarnatum. Acta Societatis Botanicorum Poloniae, 58, 51–58.
McSorley, R. (1987). Extraction of nematodes and sampling methods. In R. H. Brown & B. R. Kerry (Eds.), Principles and practises of nematode control in crops (pp. 13–47). Marrickville, NSW, Australia: AP Press.
Meher, H. C., Walia, S., & Sethi, C. L. (1988). Effect of steroidal saponins on the mobility of juveniles of Meloidogyne incognita. Indian Journal of Nematology, 18, 244–247.
Morein, B., Hu, K.-F., & Abusugra, I. (2004) Current status and potential application of ISCOMs in veterinary medicine. Advanced Drug Delivery Reviews, 56, 1367–1382.
Oleszek, W., Jurzysta, M., Ploszynski, M., Colquhoun, I. J., Price, K. R., Fenwich, G. R. (1992). Zanhic acid tridesmoside and other dominant saponins from Alfalfa (Medicago sativa L.) aerial parts. Journal of Agriculture and Food Chemistry, 40, 191–196.
Omar, S. A., Abdel-Massih, M. I., & Mohamed, B. E., (1994). Use of saponin to control the root-knot nematode Meloidogyne javanica in tomato plants. Bulletin of Faculty of Agriculture of Cairo, 45, 933–940.
Page, A. P., & Winter, A. D. (2003). Enzymes involved in the biogenesis of the nematode cuticle. Advances in Parasitology, 53, 85–148.
Pedersen, M. W., Barnes, D. K., Sorensen, E. L., Griffin, D. G., Nielson, M. W., Hill, R. R., Jr. et al. (1976). Effects of low and high saponin selection in alfalfa on agronomic and pest resistance traits and the interrelationship of these traits. Crop Science, 16, 193–199.
Pelah, D., Abramovich, Z., Markus, A., & Wiesman, Z. (2002). The use of commercial saponin from Quillaja saponaria bark as a natural larvicidal agent against Aedes aegypti and Culex pipiens. Journal of Ethnopharmacology, 81, 407–409.
Potter, S. M., Jimenez-Flores, R., Pollack, J., Lone, T. A., & Berber-Jimenez, M. D. (1993). Protein– saponin interaction and its influence on blood lipids. Journal of Agricultural and Food Chemistry, 41,1287–1291.
Raski, D. J. (1996). Dagger and needle nematodes. In R. C. Pearson & A. C. Goheen (Eds.), Compendium of grape diseases (pp. 56–59). St. Paul, MN: APS Press.
Rönnberg, B., Fekadu, M., & Morein, B. (1995). Adjuvant activity of non-toxic Quillaja saponaria Molina components for use in ISCOM matrix. Vaccine, 13, 1375–1382.
San Martin, R. (2004). Use of Quillaja saponins to control nematodes. Development of a commercial product: QL AGRI (p. 6). Pulawy, Poland: Abstract Book – International Conference on Saponins.
San Martin, R., & Magnunacelaya, J. C. (2005). Control of plant-parasitic nematodes with extracts of Quillaja saponaria. Nematology, 7, 577–585.
Tanaka, O., Tamura, Y., Masuda, H., & Mizutani, K. (1996). Application of saponins in foods and cosmetics: Saponins of Mohave yucca and Sapindus mukurossi. In G. R. Waller & K. Yamasaki (Eds.), Advances in experimental medicine and biology. Saponins used in traditional and modern medicine (pp. 565–574). New York: Plenum.
Tava, A., & Avato, P. (2006). Chemistry and biological activity of triterpene saponins from Medicago species. Natural Product Communications, 1, 1159–1180.
Tava, A., Mella, M., Avato, P., Argentieri, M. P., Bialy, Z., & Jurzysta, M. (2005). Triterpenoid glycosides from leaves of Medicago arborea L. Journal of Agricultural and Food Chemistry, 53, 9954–9965.
Tava, A., Oleszek., W., Jurzysta, M., Berardo, N., & Odoardi, M. (1993). Alfalfa saponins and sapogenins: Isolation and quantification in two different cultivars. Phytochemical Analysis, 4, 269–274.
Thorne, G., & Allen M. W. (1950). Paratylenchus hamamatus n. sp. and Xiphinema index n. sp. two nematodes associated with fig roots, with a note on Paratylenchus anceps Cobb. Proceedings of the Helminthological Society of Washington, 17, 27–35.
UNEP, United Nations Environment Programme (2000). The montreal protocol on substances that deplete the ozone layer.
Yamashita, T. T., & Viglierchio, D. R. (1987). In vitro testing for nonfumigant nematicide resistance in Xiphinema index. Revue de Nématologie, 10, 75–79.
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The authors are grateful to the Italian “Ministero dell’Università e della Ricerca Scientifica” and “Ministero delle Politiche Agricole e Forestali” for financial support.
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Argentieri, M.P., D’Addabbo, T., Tava, A. et al. Evaluation of nematicidal properties of saponins from Medicago spp.. Eur J Plant Pathol 120, 189–197 (2008). https://doi.org/10.1007/s10658-007-9207-8
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DOI: https://doi.org/10.1007/s10658-007-9207-8