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QTL mapping of fiber quality in an elite hybrid derived-RIL population of upland cotton

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Abstract

Xiangzamian 2 (XZM2) was the most widely cultivated cotton hybrid planted as F1 hybrids and as selfed F2 seeds in China before the release of transgenic Bt hybrids. By crossing two parents of XZM2, Gossypium hirsutum cv. Zhongmiansuo12 (ZMS12) and G. hirsutum acc. 8891, and through subsequent selfings, we obtained F8 and F9 populations of 180 recombinant inbred lines (RILs). A RIL population was cultivated in two cotton-growing regions in China for 2 years. The purpose of the present research was to detect quantitative trait loci (QTL) for fiber quality and provide information applicable to cotton breeding. A genetic map was constructed mainly using SSR markers. QTL controlling fiber quality traits were determined at the single-locus and two-locus levels, and genotype-by-environment interactions were analyzed. Among the main-effect QTL, a fiber length QTL qFL-D2-1 and a reflectance QTL qFR-D2-1 were simultaneously detected at two growing regions in 2 years, which suggested a high degree of stability in different environments, and might be of particular value for a marker-assisted selection (MAS) program. The results suggested that epistatic effects, as well as additive effects, of QTL play important roles in fiber quality in these RILs. In our research, the phenomenon of QTL clusters was detected in the cotton genome.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

EST:

Expression sequence tag

QTL:

Quantitative trait locus/loci

RAPD:

Random amplified polymorphic DNA

RIL:

Recombinant inbred line

SRAP:

Sequence-related amplified polymorphism

SSR:

Simple sequence repeats

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Acknowledgments

This work was supported by the grants␣from The National Natural Foundation for Outstanding␣Youth (30025029), High-tech program 863 (2002AA207006), The Changjiang Scholars and Innovative Research Team in University and The Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE, China. The authors show gratitude to Prof. Dr. Jun Zhu in Zhejiang University and Mr. XH He in NAU for their assistance in data analysis.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, 210095, China

    Baohua Wang, Wangzhen Guo, Xiefei Zhu, Yaoting Wu, Naitai Huang & Tianzhen Zhang

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  1. Baohua Wang
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  2. Wangzhen Guo
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  3. Xiefei Zhu
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  4. Yaoting Wu
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  5. Naitai Huang
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  6. Tianzhen Zhang
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Correspondence to Tianzhen Zhang.

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Wang, B., Guo, W., Zhu, X. et al. QTL mapping of fiber quality in an elite hybrid derived-RIL population of upland cotton. Euphytica 152, 367–378 (2006). https://doi.org/10.1007/s10681-006-9224-2

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  • DOI: https://doi.org/10.1007/s10681-006-9224-2

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