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The development of a genetic linkage map of blackcurrant (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits

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Abstract

The first genetic linkage map of blackcurrant (Ribes nigrum L.) was constructed using AFLP, SSR (genomic and EST-derived) and SNP markers, in a mapping population derived from two diverse breeding clones of blackcurrant from the SCRI breeding programme. Cluster analysis of the population revealed that the individuals within the population formed two distinct sub-populations, with segregation ratios consistent with one sub-population having the two intended parents, and the other being selfed segregants. The latter sub-population improves the map by providing a more informative estimate of recombination frequency than the crossed sub-population for some marker configurations, and also revealed the presence of two unlinked loci affecting viability. Several important phenological, agronomic and fruit quality traits were evaluated in the mapping population, and QTLs affecting these are located on the linkage map. This provides a framework for the development of marker-assisted breeding strategies for blackcurrant, to improve breeding efficiency and time to cultivar.

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References

  • Atkinson C, Sunley R, Jones H, Brennan R, Darby P (2004) Winter Chill in Fruit. UK Department of Food and Rural Affairs Report No. CTC 0206

  • Bradshaw JE, Hackett CA, Meyer RC, Milbourne DM, McNicol JW, Phillips MS, Waugh R (1998) Identification of AFLP and SSR markers associated with quantitative resistance to Globodera pallida (Stone) in tetraploid potato (Solanum tuberosum subsp. tuberosum) with a view to marker-assisted selection. Theor Appl Genet 97:202–210

    Article  Google Scholar 

  • Boccorh RK, Paterson A, Piggott JR (1998) Factors affecting sugars and organic acids in blackcurrant concentrates. Z Lebensm Unters Forsch A 206:273–278

    Article  CAS  Google Scholar 

  • Brennan RM (2006) Currants and Gooseberries Ribes species Saxifragaceae. In: Janick J (ed) Encyclopedia of Fruit and Nut Crops. CABI, Cambridge MA (in press)

  • Brennan RM (1996) Currants and gooseberries. In: Janick J, Moore JN (eds) Fruit breeding, vol II: vine and small fruits crops. Wiley, New York

    Google Scholar 

  • Brennan R, Jorgensen L, Woodhead M, Russell J (2002) Development and characterisation of SSR markers in Ribes species. Mol Ecol Notes 2:327–330

    Article  CAS  Google Scholar 

  • Chiche J, Brown SC, Leclerc J-C, Siljak-Yakovlev S (2003) Genome size, heterochromatin organisation, and ribosomal gene mapping in four species of Ribes. Can J Bot 81:1049–1057

    Article  CAS  Google Scholar 

  • Cho RJ, Mindrinos M, Richards DR, Sapolsky RJ, Anderson M, Drenkar E, Dewdney J, Reuber TL, Stammers M, Federerspiel N (1999) Genome-wide mapping with biallelic markers in Arabidopsis thaliana. Nature Genet 23:203–20

    Article  PubMed  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Digby PGN, Kempton RA (1987) Multivariate analysis of ecological communities. Chapman & Hall, London, New York

    Google Scholar 

  • Dirlewanger E, Graziano E, Joobeur T, Garriga-Calderé F, Cosson P, Howad W, Arùs P (2004) Comparative mapping and marker-assisted selection in Rosaceae fruit crops. Proc Nat Acad Sci 101:9891–9896

    Article  PubMed  CAS  Google Scholar 

  • Edwards KJ, Barker JHA, Daly A, Jones C, Karp A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20:758–760

    PubMed  CAS  Google Scholar 

  • Engler A, Prantl K (1891) Ribesioideae. Naturl Pflanzenfam 3:97–142

    Google Scholar 

  • Graham J, Smith K, MacKenzie K, Jorgensen L, Hackett C, Powell W (2004) The construction of a genetic linkage map of red raspberry (Rubus idaeus subsp. idaeus) based on AFLPs, genomic-SSR and EST-SSR markers. Theor Appl Genet 109:704–749

    Article  CAS  Google Scholar 

  • Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudotestcross mapping strategy and RAPD markers. Genetics 137:1121–1137

    PubMed  CAS  Google Scholar 

  • Griffin TJ, Smith LM (2000) Single-nucleotide polymorphism analysis by MALDI-TOF mass spectrometry. Trends Biotechnol 18:77–84

    Article  PubMed  CAS  Google Scholar 

  • Hackett CA, Wachira FN, Paul S, Powell W, Waugh R (2000) Construction of a genetic linkage map for Camellia sinensis (tea). Heredity 85:346–355

    Article  PubMed  CAS  Google Scholar 

  • Hale ML, Bevan RM, Wolff K (2001) New polymorphic microsatellite markers for the red squirrel (Sciurus vulgaris) and their applicability to the grey squirrel (S. canadensis) Mol Ecol Notes 1:47–49

    Article  CAS  Google Scholar 

  • Hemmat M, Weeden NF, Manganaris AG, Lawson DM (1994) Molecular marker linkage map for apple. J Heredity 85:4–11

    CAS  Google Scholar 

  • Janczewski E de (1907) Monographie des groseilliers, Ribes L. Mémoires de la Société de physique et d’histoire naturelle de Genève 35:199–517

    Google Scholar 

  • Knight RL, Keep E, Briggs JB, Parker JH (1974) Transference of resistance to blackcurrant gall mite, Cecidophyopsis ribis, from gooseberry to blackcurrant. Ann Appl Biol 76:123–130

    Article  Google Scholar 

  • Lanham PG, Brennan RM (1999) Genetic characterization of gooseberry (Ribes grossularia subgenus Grossularia) germplasm using RAPD, ISSR and AFLP markers. J Hort Sci Biotech 74:361–366

    CAS  Google Scholar 

  • Lanham PG, Korycinska A, Brennan RM (2000) Genetic diversity within a secondary gene pool for Ribes nigrum L. revealed by RAPD and ISSR markers. J Hort Sci Biotech 75:371–375

    CAS  Google Scholar 

  • Lister CE, Wilson PE, Sutton KH, Morrison SC (2002) Understanding the health benefits of blackcurrants. Acta Hort 585:443–449

    CAS  Google Scholar 

  • Lohdi MA, Daly MJ, Ye G-N, Weeden NF, Reisch BI (1995) A molecular marker based linkage map of Vitis. Genome 38:786–794

    Google Scholar 

  • Maliepaard C, Jansen J, Van Ooijen JW (1997) Linkage mapping in a full-sib family of an outbreeding plant species: overview and consequences for applications. Genet Res 70:237–250

    Article  Google Scholar 

  • Milligan BG (1992) Plant DNA isolation. In: Hoelzed AR (ed) Molecular genetics of populations: a practical approach. IRL Press, Oxford, UK

    Google Scholar 

  • Roach FA (1985) Cultivated fruits of Britain. Their origin and history. Blackwell, Oxford

    Google Scholar 

  • Rohde A, Howe GT, Olsen JE, Moritz T, Van Montagu M, Juntilla O, Boerjan W (2000) Molecular aspects of bud dormancy in trees. In: Jain SM, Minocha SC (eds) Molecular biology of woody plants vol 1. Kluwer, Amsterdam

    Google Scholar 

  • Rozen S, Skaletsky HJ (1996) PRIMER 3. Code available at http://www-genome.wi.mit.edu/genome_software/other/primer3.html

  • Sargent DJ, Davis TM, Tobutt KR, Wilkinson MJ, Battey NH, Simpson DW (2004) A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria. Theor Appl Genet 109:1385–1391

    Article  PubMed  CAS  Google Scholar 

  • Scott KD (2001) Microsatellites derived from ESTs and their comparison with those derived by other methods. In: Henry RY (ed) Plant genotyping: the DNA fingerprinting of plants. CABI, Cambridge, MA

    Google Scholar 

  • Shaklady J (1969) Fruit juices and fruit juice beverages. In: Woolen A (ed) Food industries manual, 20th edn. Leonard Hill, London

    Google Scholar 

  • Stam P, Van Ooijen JW (1995) JoinmapTM version 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen

    Google Scholar 

  • Taylor J (1989) Colour stability of blackcurrant (Ribes nigrum L.) juice. J Sci Food Agric 49:487–491

    Article  CAS  Google Scholar 

  • Van Ooijen JW, Maliepaard C (1996) MapQTLTM version 3.0: software for the calculation of QTL positions on genetic linkage maps. CPRO-DLO, Wageningen

    Google Scholar 

  • Walker PW, Gordon SL, Brennan RM, Hancock RD (2006) A high-throughput monolithic HPLC method for rapid vitamin C phenotyping of berry fruit. Phytochem Anal 17:284–290

    Article  PubMed  CAS  Google Scholar 

  • Woodhead M, Taylor MA, Brennan R, McNicol RJ, Davies HV (1998) Cloning and characterisation of the cDNA clones of five genes that are differentially expressed during ripening in the fruit of blackcurrant (Ribes nigrum L.). J Plant Physiol 153:381–393

    CAS  Google Scholar 

  • Woodhead M, Russell J, Squirell J, Hollingsworth PM, Cardle L, Ramsay L, Gibby M, Powell W (2003) Development of EST-SSRs from the alpine lady-fern Athyrium distentifolium. Mol Ecol Notes 3:287–290

    Article  CAS  Google Scholar 

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Acknowledgements

Financial support for this work was provided by the Scottish Executive Environment and Rural Affairs Department. The authors also thank John Bradshaw (SCRI) for helpful comments on the manuscript, and Linda Cardle and Claire Booth (SCRI) for assistance with sequence analysis and genotyping.

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Authors and Affiliations

  1. Fruit Breeding Group, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK

    Rex Brennan, Linzi Jorgensen, Mary Woodhead, Sandra Gordon & Joanne Russell

  2. Biomathematics and Statistics Scotland, Dundee Unit, Invergowrie, Dundee, DD2 5DA, Scotland, UK

    Christine Hackett

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  1. Rex Brennan
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  2. Linzi Jorgensen
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  3. Christine Hackett
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  4. Mary Woodhead
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  5. Sandra Gordon
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  6. Joanne Russell
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Correspondence to Rex Brennan.

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Brennan, R., Jorgensen, L., Hackett, C. et al. The development of a genetic linkage map of blackcurrant (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits. Euphytica 161, 19–34 (2008). https://doi.org/10.1007/s10681-007-9412-8

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  • DOI: https://doi.org/10.1007/s10681-007-9412-8

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