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Somatic hybrids of vegetable brassicas as source for new resistances to fungal and virus diseases

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Abstract

Somatic hybrids were produced by PEG-induced symmetric and asymmetric protoplast fusions in order to transfer resistance to Alternaria brassicicola, A. brassicae, Phoma lingam, Plasmodiophora brassicae and Turnip mosaic virus (TuMV) into Brassica oleracea var. capitata (cv. ‘Toskama’) and botrytis (cv. ‘Korso’). As resistance donors, ten species belonging to several genera of the family Brassicaceae including wild relatives were used. Of 2,189 plants (somatic hybrids, partially in vitro cloned) tested, 1,616 (73.8%) were resistant against at least one of the pathogens, indicating that, mostly, a successful resistance transfer has taken place. Five hundred and twenty-two hybrids showed multiple resistances to two, three and, in a single case, to four pathogens. Irrespective of the donor parents used in the fusion, a broad variability in symptom manifestation ranging from 0 (without symptoms) to 9 (highly susceptible) could be observed. With regard to the Alternaria pathogens, Sinapis alba, B. nigra and B. juncea were the most effective resistance donors, whereas fusions with Raphanus sativus resulted in the most hybrids with resistance to clubroot and TuMV. As could be shown especially in asymmetric fusions with S. alba, Barbarea vulgaris and Hesperis matronalis, transferred resistance to a pathogen may not correspond with resistance exhibited by the donor parent. Some combinations in which both parents were highly susceptible, e.g. R. sativus (+) B. oleracea var. capitata, yielded hybrids that exhibited strong resistance, e.g. to A. brassicicola, revealing that a new type of resistance might be occurring. With regard to the Alternaria pathogens, resistance expression was very unstable. Many hybrids into which (also variable) resistance of some donors, such as B. vulgaris, S. alba and B. carinata, was transferred became as highly susceptible as those of which the fusion parents did not show any resistance reaction (e.g. R. sativus). For reliable characterization of the resistance response, hybrids should be subjected to several resistance tests during growth period of the host, at least until flowering.

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Acknowledgments

We would like to thank Mrs. E. Zjaba, A. Krüger, M. Malorny, and R. Dippe for excellent technical assistance.

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  1. Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural and Fruit Crops, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Paul Scholze, Reiner Krämer, Ulrich Ryschka, Evelyn Klocke & Günter Schumann

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  1. Paul Scholze
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  2. Reiner Krämer
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Correspondence to Reiner Krämer.

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Scholze, P., Krämer, R., Ryschka, U. et al. Somatic hybrids of vegetable brassicas as source for new resistances to fungal and virus diseases. Euphytica 176, 1–14 (2010). https://doi.org/10.1007/s10681-010-0205-0

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