Skip to main content
Log in

Thermotolerance and Related Antioxidant Enzyme Activities Induced by Heat Acclimation and Salicylic Acid in Grape (Vitis vinifera L.) Leaves

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Thermotolerance and related antioxidant enzyme activities induced by both heat acclimation and exogenous salicylic acid (SA) application were studied in grapevine (Vitis vinifera L. cv. Jingxiu). Heat acclimation and exogenous SA application induced comparable changes in thermotolerance, ascorbic acid (AsA), glutathione (GSH), and hydrogen peroxide (H2O2) concentrations, and in activities of the antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), ascorbic peroxidase (APX) and catalase (CAT) in grape leaves. Within 1 h at 38 °C, free SA concentration in leaves rose from 3.1 μg g−1 FW to 19.1 μg g−1 FW, then sharply declined. SA application and heat acclimation induced thermotolerance were related to changes of antioxidant enzyme activities and antioxidant concentration, indicating a role for endogenous SA in heat acclimation in grape leaves.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbic acid

CAT:

catalase

GR:

glutathione reductase

GSH:

glutathione

POD:

peroxidase

SA:

salicylic acid

SOD:

superoxide dismutase

References

  • N. Banzet C. Richaud Y. Deveaux M. Kazmaier J. Gagnon C. Triantaphylides (1998) ArticleTitleAccumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells Plant J. 13 519–527 Occurrence Handle10.1046/j.1365-313X.1998.00056.x Occurrence Handle1:CAS:528:DyaK1cXitFyrs7w%3D Occurrence Handle9680997

    Article  CAS  PubMed  Google Scholar 

  • P.F. Beers I.W. Sizer (1952) ArticleTitleA spectrophotometric assay for measuring the breakdown of hydrogen peroxide by catalase J. Biol. Chem. 195 133–138 Occurrence Handle1:CAS:528:DyaG38Xjt12juw%3D%3D Occurrence Handle14938361

    CAS  PubMed  Google Scholar 

  • O. Borsani V. Valpuesta M.A. Botella (2001) ArticleTitleEvidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis plants Plant Physiol. 126 1024–1030 Occurrence Handle10.1104/pp.126.3.1024 Occurrence Handle1:CAS:528:DC%2BD3MXlsVartbY%3D Occurrence Handle11457953

    Article  CAS  PubMed  Google Scholar 

  • C. Bowler W. Camp ParticleVan M. Montagu ParticleVan D. Inze (1994) ArticleTitleSuperoxide dismutase in plants Crit. Rev. Plant Sci. 13 199–218 Occurrence Handle1:CAS:528:DyaK2cXmvFOis7w%3D

    CAS  Google Scholar 

  • H.H. Chen Z.Y. Shen P.H. Li (1982) ArticleTitleAdaptability of crop plants to high temperature stress Crop Sci. 22 719–725

    Google Scholar 

  • Z. Chen J.R. Riciglian D.F. Klessig (1993) ArticleTitlePurification and characterization of a soluble salicylic acid binding protein from tobacco Proc. Natl. Acad. Sci. USA 90 9533–9537 Occurrence Handle1:CAS:528:DyaK3sXmsVeqs7k%3D Occurrence Handle8415736

    CAS  PubMed  Google Scholar 

  • U. Conrath H. Silva D.F. Klesslg (1997) ArticleTitleProtein dephosphorylation mediates salicylic acid-induced expression of PR-1 genes in tobacco Plant J. 11 747–757 Occurrence Handle10.1046/j.1365-313X.1997.11040747.x Occurrence Handle1:CAS:528:DyaK2sXjs1Clurk%3D

    Article  CAS  Google Scholar 

  • J.F. Dat H. Lopez-Delgado C.H. Foyer I.M. Scott (1998a) ArticleTitleParallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard plants Plant Physiol. 116 1351–1357 Occurrence Handle10.1104/pp.116.4.1351 Occurrence Handle1:CAS:528:DyaK1cXisFyqsbg%3D

    Article  CAS  Google Scholar 

  • J.F. Dat C.H. Foyer I.M. Scott (1998b) ArticleTitleChange in salicylic acid and antioxidants during induced thermotolerance in mustard plants Plant Physiol. 118 1445–1456 Occurrence Handle10.1104/pp.118.4.1455

    Article  Google Scholar 

  • C.K. Ding C.Y. Wang K.C. Gross D.L. Smith (2002) ArticleTitleJasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to cold injury in tomato fruit Planta 214 895–901 Occurrence Handle10.1007/s00425-001-0698-9 Occurrence Handle1:CAS:528:DC%2BD38Xis1Sjsr0%3D Occurrence Handle11941466

    Article  CAS  PubMed  Google Scholar 

  • J. Duner J. Shah D.F. Klessig (1997) ArticleTitleSalicylic acid and disease resistance in plants Trends Plant Sci. 7 266–274

    Google Scholar 

  • J.G. Foster J.L. Hess (1980) ArticleTitleResponse of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen Plant Physiol. 66 482–487 Occurrence Handle1:CAS:528:DyaL3cXlvVartb0%3D

    CAS  Google Scholar 

  • C.H. Foyer J. Rowell D. Walker (1983) ArticleTitleMeasurements of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination Planta 157 239–244 Occurrence Handle10.1007/BF00405188 Occurrence Handle1:CAS:528:DyaL3sXhvFGjsbc%3D

    Article  CAS  Google Scholar 

  • C.H. Foyer H. Lopez-Delgado J.F. Dat I.M. Scott (1997) ArticleTitleHydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signalling Physiol. Plant. 100 241–254 Occurrence Handle10.1034/j.1399-3054.1997.1000205.x Occurrence Handle1:CAS:528:DyaK2sXkvFersLc%3D

    Article  CAS  Google Scholar 

  • T. Gecheva I. Gadjeva F. Breusegemb ParticleVan D. Inzéb S. Dukiandjieva V. Tonevaa I. Minkova (2002) ArticleTitleHydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes Cell. Mol. Life Sci. 59 708–714

    Google Scholar 

  • O.W. Griffiths (1980) ArticleTitleDetermination of glutathione and glutathione disulphide using glutathione reductase and 2-vinypyridine Anal. Biochem. 106 207–212

    Google Scholar 

  • R. Hammerschmidt E.M. Nuckles J. Kuc (1982) ArticleTitleAssociation of enhanced peroxide activity with induced systemic resistance of cucumber to Colletotrichum lagenarium Physiol. Plant Pathol. 20 73–82 Occurrence Handle1:CAS:528:DyaL38XhtF2hsLw%3D

    CAS  Google Scholar 

  • T. Janda G. Szalai L. Tari E. Paldi (1999) ArticleTitleHydroponic treatment with salicylic acid decreases the effects of cold injury in maize (Zea mays L.) plants Planta 208 175–180 Occurrence Handle10.1007/s004250050547 Occurrence Handle1:CAS:528:DyaK1MXjtFejt7c%3D

    Article  CAS  Google Scholar 

  • T. Kawano N. Sahashi K. Takahashi N. Uozumi S. Muto (1998) ArticleTitleSalicylic acid induces extracellular superoxide generation followed by an increase in cytosolic calcium ion in tobacco suspension culture: the earliest events in salicylic acid signal transduction Plant Cell Physiol. 39 721–730 Occurrence Handle1:CAS:528:DyaK1cXkslGisbc%3D

    CAS  Google Scholar 

  • S. Karpinski C. Escobar B. Karpinska G. Creissen P.M. Mullineaux (1997) ArticleTitlePhotosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress Plant Cell 9 627–640 Occurrence Handle10.1105/tpc.9.4.627 Occurrence Handle1:CAS:528:DyaK2sXivFCrs7k%3D Occurrence Handle9144965

    Article  CAS  PubMed  Google Scholar 

  • T.E. Kraus R.A. Fletcher (1994) ArticleTitlePaclobutrazol protects wheat plants from heat and paraquat injury. Is detoxification of active oxygen involved? Plant Cell Physiol. 35 45–52 Occurrence Handle1:CAS:528:DyaK2cXitFSlu7k%3D

    CAS  Google Scholar 

  • A. Kubo H. Saji K. Tanaka N. Kondo (1995) ArticleTitleExpression of Arabidopsis cytosolic ascorbate peroxidase gene in response to ozone or sulphur dioxide Plant Mol. Biol. 29 479–489 Occurrence Handle10.1007/BF00020979 Occurrence Handle1:CAS:528:DyaK28Xit1aqtA%3D%3D Occurrence Handle8534847

    Article  CAS  PubMed  Google Scholar 

  • G. Kocsya G. Galibaa C. Brunoldb (2001) ArticleTitleRole of glutathione in adaptation and signaling during chilling and cold acclimation in plants Physiol. Plant 113 158–164

    Google Scholar 

  • J. Levitt (1980) Responses of Plants to Environmental Stresses: WaterRadiation, Salt and Other Stresses EditionNumber2 Academic Press New York, USA 25–280

    Google Scholar 

  • H. Lopez-Delgado J.F. Dat C.H. Foyer I.M. Scott (1998) ArticleTitleInduction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2 J. Exp. Bot. 49 713–720 Occurrence Handle10.1093/jexbot/49.321.713 Occurrence Handle1:CAS:528:DyaK1cXivV2ntLc%3D

    Article  CAS  Google Scholar 

  • R. Mittler B.A. Zilinskas (1992) ArticleTitleMolecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase J. Biol. Chem. 262 21802–21807

    Google Scholar 

  • Y. Nakano K. Asada (1981) ArticleTitleHydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts Plant Cell Physiol. 22 867–880 Occurrence Handle1:CAS:528:DyaL3MXltFWqur0%3D

    CAS  Google Scholar 

  • G. Noctor A.C.M. Arisi L. Jouanin K.J. Kunert H. Rennenberg C.H. Foyer (1998) ArticleTitleGlutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transgenic plants J. Exp. Bot. 49 623–647 Occurrence Handle1:CAS:528:DyaK1cXivV2ntLw%3D

    CAS  Google Scholar 

  • D. O’Kane V. Gill P. Boyd R. Burdon (1996) ArticleTitleChilling, oxidative stress and antioxidant response in Arabidopsis thallana callus Planta 198 371–377 Occurrence Handle1:CAS:528:DyaK28XkvVeqsA%3D%3D Occurrence Handle8717134

    CAS  PubMed  Google Scholar 

  • B.D. Patterson E.A. Machae I.B. Ferguson (1984) ArticleTitleEstimation of hydrogen peroxide in plant extracts using titanium(IV) Anal. Biochem. 139 487–492 Occurrence Handle10.1016/0003-2697(84)90039-3 Occurrence Handle1:CAS:528:DyaL2cXkt1SjtLs%3D Occurrence Handle6476384

    Article  CAS  PubMed  Google Scholar 

  • T.K. Palva M. Hurting P. Saindrenan (1994) ArticleTitleSalicylic acid induced resistance to Erwinia carotovora subsp. cartovora in tobacco Mol. Plant Microbe Interact. 7 356–363 Occurrence Handle1:CAS:528:DyaK2cXltlSisLo%3D

    CAS  Google Scholar 

  • M.V. Rao G. Paliyath P. Ormrod D.P. Murr C.B. Watkins (1997) ArticleTitleInfluence of salicylic acid on H2O2 production, oxidative stress and H2O2-metabolizing enzymes Plant Physiol. 115 137–149 Occurrence Handle10.1104/pp.115.1.137 Occurrence Handle1:CAS:528:DyaK2sXmt1yntLc%3D Occurrence Handle9306697

    Article  CAS  PubMed  Google Scholar 

  • J.B. Rasmussaen R. Hammerschmidt M.N. Zook (1991) ArticleTitleSystemic induction of salicylic acid accumulation in cucumber after inoculation with Pseudomonas syringae pv Syringae Plant Physiol. 97 1342–1347

    Google Scholar 

  • R.C. Stewart J.D. Bewley (1980) ArticleTitleLipid peroxidation associated with accelerated aging of soybean axes Plant Physiol. 65 245–248 Occurrence Handle1:CAS:528:DyaL3cXht1Git7c%3D

    CAS  Google Scholar 

  • Y.K. Sharma J. Leon l. Raskin K.R. Davis (1996) ArticleTitleOzone induced responses in Arabidopsis thaliana – the role of salicylic acid in the accumulation of defence-related transcripts and induced resistance Proc. Natl. Acad. Sci. USA 93 5099–5104 Occurrence Handle1:CAS:528:DyaK28XjtVKlur0%3D Occurrence Handle8643534

    CAS  PubMed  Google Scholar 

  • K. Shirasu H. Nakajima V.K. Rajasekhar R.A. Dixon C. Lamb (1997) ArticleTitleSalicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signals in the activation of defense mechanisms Plant Cell 9 261–270 Occurrence Handle10.1105/tpc.9.2.261 Occurrence Handle1:CAS:528:DyaK2sXhslGrtbs%3D Occurrence Handle9061956

    Article  CAS  PubMed  Google Scholar 

  • E. Tasgin O Atici B. Nalbantoglu (2003) ArticleTitleEffects of salicylic acid and cold on freezing tolerance in winter wheat leaves Plant Growth Regul. 41 231–236 Occurrence Handle1:CAS:528:DC%2BD3sXps1amsrw%3D

    CAS  Google Scholar 

  • W. Camp ParticleVan M.V. Montagu D. Inze (1998) ArticleTitleH2O2NO: redox signals in disease resistance Trends Plant Sci. 3 330–334

    Google Scholar 

  • C.Y. Wang (1995) ArticleTitleTemperature preconditioning affects glutathione content and glutathione reductase activity in chilled zucchini squash J. Plant Physiol. 145 148–152 Occurrence Handle1:CAS:528:DyaK2MXjsVGkt78%3D

    CAS  Google Scholar 

  • L.J. Wang W.D. Huang F.Y. Yu (2001) ArticleTitleEffect of elevated temperature on transportion and distribution of 14C-salicylic acid in grape seedlings Acta Phytophys. Sinica 27 129–134 Occurrence Handle1:CAS:528:DC%2BD3MXotVCqs78%3D

    CAS  Google Scholar 

  • N. Yalpani A.J. Enyedi J. Leon I. Raskin (1994) ArticleTitleUltraviolet light and ozone stimulate accumulation of salicylic acidpathogenesis-related proteins and virus resistance in tobacco Planta 193 372–376 Occurrence Handle10.1007/BF00201815 Occurrence Handle1:CAS:528:DyaK2cXitl2iurc%3D

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Shao-Hua Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, LJ., Li, SH. Thermotolerance and Related Antioxidant Enzyme Activities Induced by Heat Acclimation and Salicylic Acid in Grape (Vitis vinifera L.) Leaves. Plant Growth Regul 48, 137–144 (2006). https://doi.org/10.1007/s10725-005-6146-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-005-6146-2

Keywords

Navigation