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Altered expression of CDX-2, PDX-1 and mucin core proteins in “Ulcer-associated cell lineage (UACL)” in Crohn’s disease

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Abstract

The ulcer-associated cell lineage (UACL) induced at the site of ileac chronic ulceration in Crohn’s disease has been reported to show histological differentiation resembling gastric pyloric mucosa. To clarify the significance of homeobox gene-encoded transcription factors in the formation of the UACL in Crohn’s disease, we investigated the immunohistochemical expression of gastrointestinal mucins (MUC5AC for gastric surface mucous cells; MUC6 for gastric gland mucous cells, and MUC2 for intestinal goblet cells) and homeobox gene-encoded transcription factors (CDX-2 for intestinal mucosa and PDX-1 for pyloric mucosa) in the UACL. The analysis was undertaken on ileal mucosa obtained from ileal resections performed in 19 patients with active Crohn’s disease of the small bowel. The UACL was observed in nine patients. In the UACL, expression of mucous cells with a foveolar-structure showed immunoreactivity to MUC5AC, and the mucous cells with a glandular structure showed immunoreactivity to MUC6, and the expression of MUC2 was decreased. In addition, we detected the decreased expression of CDX-2 along with the increased expression of PDX-1 in the UACL. The UACL showed histological differentiation simulating gastric pylori mucosa. The down-regulation of CDX-2 and the up-regulation of PDX-1 could be an important mechanism in the induction of the UACL.

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Acknowledgments

We gratefully thank Professor Christopher V. E. Wright (Vanderbilt Developmental Biology Program, Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA) for the gift of critical antibody reagent, Professor Tsutomu Katsuyama (Department of Laboratory Medicine, Shinshu University School of Medicine) and Professor Kendo Kiyosawa (Second Department of Internal Medicine, Shinshu University School of Medicine) for their advice and encouragement.

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Authors and Affiliations

  1. Department of Medicine, Gastroenterology, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan

    Yasunori Kaneko

  2. Department of Laboratory Medicine, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan

    Takamichi Nakamura

  3. Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan

    Masayoshi Hayama & Hiroyoshi Ota

  4. Department of Laboratory Medicine, Nagano Municipal Hospital, 1333-1 Tomitake, Nagano, Japan

    Noriko Hosaka

  5. Department of Endoscopy, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Japan

    Taiji Akamatsu

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  1. Yasunori Kaneko
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  2. Takamichi Nakamura
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  3. Masayoshi Hayama
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  4. Noriko Hosaka
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  5. Taiji Akamatsu
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  6. Hiroyoshi Ota
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Correspondence to Hiroyoshi Ota.

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Kaneko, Y., Nakamura, T., Hayama, M. et al. Altered expression of CDX-2, PDX-1 and mucin core proteins in “Ulcer-associated cell lineage (UACL)” in Crohn’s disease. J Mol Hist 39, 161–168 (2008). https://doi.org/10.1007/s10735-007-9149-7

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  • DOI: https://doi.org/10.1007/s10735-007-9149-7

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