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Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

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Abstract

Probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine IPEC-J2 enterocytes to oxidative stress remained to be elucidated. The immunmodulatory effect of five bacterial strains (Lactobacillus plantarum 2142, Lactobacillus casei Shirota, Bifidobacterium animalis subsp. lactis BB-12, Bacillus amyloliquefaciens CECT 5940 and Enterococcus faecium CECT 4515) on 1 mM peroxide-triggered upregulation of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-α) level was screened by q RT-PCR. Our data revealed that spent culture supernatant (SCS) of L. plantarum 2142 had significant lowering effect on IL-8 and TNF-α level with concomitant promoting activity on protective Hsp70 gene expression. According to our results, lactic acid (racemic, d- and l-lactic acid) and acetic acid produced by lactobacilli had no protective effect in quenching upregulation of proinflammatory cytokines. Furthermore, L. plantarum 2142-specific supernatant peptides were detected by gel electrophoresis and capillary zone electrophoresis.

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ACKNOWLEDGMENT

The research described here has been supported by the Hungarian Scientific Research Fund (grant OTKA nos. 76133 and 100701). We are indebted to Dr. Jody Gookin and Dr. Stephen Stauffer, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA for providing IPEC-J2 cells and for the valuable advice on handling them. PCR product sequencing support from Dr. Balázs Gereben (Institute of Experimental Medicine of the Hungarian Academy of Sciences, Laboratory of Endocrine Neurobiology) is also acknowledged. We also would like to thank Dr. Éva Gelencsér, Dr. Emőke Németh-Szerdahelyi and Katalin Háder-Sólyom (Central Food Research Institute, Food Safety Department, Unit of Biology, Budapest, Hungary) for their extensive support in peptide electrophoretic studies.

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  1. Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences, Szent István University, István u. 2, 1078, Budapest, Hungary

    Erzsebet Paszti-Gere, Krisztina Szeker, Edina Csibrik-Nemeth, Rita Csizinszky, Andras Marosi, Orsolya Palocz, Orsolya Farkas & Peter Galfi

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  1. Erzsebet Paszti-Gere
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Correspondence to Erzsebet Paszti-Gere.

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Paszti-Gere, E., Szeker, K., Csibrik-Nemeth, E. et al. Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line. Inflammation 35, 1487–1499 (2012). https://doi.org/10.1007/s10753-012-9462-5

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