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Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

  • ANDROLOGY
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Abstract

Objective

To evaluate the sperm’s chromatin quality in couples with spontaneous recurrent abortion.

Methods

Thirty couples with spontaneous recurrent abortion (case group) and 30 fertile couples (control group) referring to Zeinabieh Gynecology clinic of Shiraz were included. Semen samples were collected for each participant and were used for standard semen analysis and sperm nuclear maturity tests including Chromomycin A3 (CMA3), Aniline Blue (AB) staining and Acridine Orange (AO) test (by light microscopy).

Result

Patients in case group had significantly higher percentage of CMA3 (p < 0.001) and AB (p < 0.001) positive spermatozoa compared to controls. However AO results did not differ significantly between groups (p = 0.656). Sperm morphology and progressive motility were negatively correlated with CMA3 (p = 0.001 and p = 0.043) and AB (p = 0.015 and p = 0.031) respectively.

Conclusion

Evaluation of the sperm’s quality via CMA3 and AB staining could be considered as one of the complementary tests of semen analysis for assessment of male factor in couples with spontaneous recurrent abortion.

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Acknowledgments

The authors wish to thank all the couples who participated in this study.

Author information

Authors and Affiliations

  1. Shiraz University of Medical Sciences, Shiraz, Iran

    Talieh Kazerooni, Nasrin Asadi, Leila Jadid & Jaleh Zolghadr

  2. IVF Center of Shiraz, Shiraz, Iran

    Marjaneh Kazerooni & Alireza Ghanadi

  3. Fasa University of Medical Sciences, Fasa, Iran

    Fariborz Ghaffarpasand

  4. New Mexico Tech University, Socorro, New Mexico, USA

    Yasaman Kazerooni

  5. PO Box: 7173646199, #103 – Biat Alley – Shuride Shirazi Street, Shiraz, Fars, Iran

    Fariborz Ghaffarpasand

Authors
  1. Talieh Kazerooni
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  2. Nasrin Asadi
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  3. Leila Jadid
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  4. Marjaneh Kazerooni
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  5. Alireza Ghanadi
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  6. Fariborz Ghaffarpasand
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  7. Yasaman Kazerooni
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  8. Jaleh Zolghadr
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Corresponding author

Correspondence to Fariborz Ghaffarpasand.

Additional information

The work was performed in

Infertility Research Center, Department of Obstetrics and Gynecology, Zeinabieh Hospital, Shiraz University of Medical Science, Shiraz, Iran.

Capsule

Evaluation of the sperm’s quality via CMA3 and AB staining is useful for assessment of male factor in couples with spontaneous recurrent abortion.

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Kazerooni, T., Asadi, N., Jadid, L. et al. Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet 26, 591–596 (2009). https://doi.org/10.1007/s10815-009-9361-3

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  • DOI: https://doi.org/10.1007/s10815-009-9361-3

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