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The anatase phase of nanotopography titania plays an important role on osteoblast cell morphology and proliferation

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Abstract

The surface properties of biomaterials play a vital role in cell morphology and behaviors such as cell adhesion, migration, proliferation and differentiation. Three different crystal phases of titania film (rutile, anatase and amorphous titania) with similar roughness were successfully synthesized by DC reactive magnetron sputtering. The surface roughness of each film was about 8–10 nm. Primary rat osteoblasts were used to observe changes in morphology and to evaluate cell behavior at the film surface. The number of the osteoblasts on anatase film was significantly higher than rutile and amorphous films after 36 and 72 h incubation. More importantly, synthesis of alkaline phosphatase was significantly greater by osteoblasts cultured on anatase film than on rutile and amorphous films after 7 and 14 days. In addition, the cells grown on the anatase phase film had the largest spreading area; the actin filaments in cells with regular directions were well defined and fully spreaded. The results indicate that the anatase phase of titania with nanoscale topography yield the best biological effects for cell adhesion, spreading, proliferation and differentiation. There are strong therapeutic prospects for this biomaterial film for osteoblast proliferation, with possible applications for orthopedic and dental implant.

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Acknowledgements

The authors would like to thank Mrs. QH Lu (Analysis and Measurement Center of Shanghai JiaoTong University) for AFM measurement and modification of the manuscript. The project was financially supported by the National Natural Science Foundation of China (30572053) and Shanghai Science and Technology Committee (0452nm063 and 0652nm018).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Jie He, Xiaojian Zhou, Pengbo Wan & Wantao Chen

  2. Department of Physics, Shanghai Jiao Tong University, Shanghai, 200030, China

    Wei Zhou & Xiaoxia Zhong

  3. Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China

    Xiaojian Zhou, Xiuli Zhang & Wantao Chen

  4. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai, 200030, China

    Bangshang Zhu

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  1. Jie He
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  2. Wei Zhou
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  3. Xiaojian Zhou
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  4. Xiaoxia Zhong
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  5. Xiuli Zhang
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  6. Pengbo Wan
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  7. Bangshang Zhu
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  8. Wantao Chen
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Correspondence to Wantao Chen.

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J. He and W. Zhou contributed to this work equally.

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He, J., Zhou, W., Zhou, X. et al. The anatase phase of nanotopography titania plays an important role on osteoblast cell morphology and proliferation. J Mater Sci: Mater Med 19, 3465–3472 (2008). https://doi.org/10.1007/s10856-008-3505-3

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  • DOI: https://doi.org/10.1007/s10856-008-3505-3

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