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ANT2 Isoform Required for Cancer Cell Glycolysis

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Abstract

The three adenine nucleotide translocator ({ANT1} to {ANT3}) isoforms, differentially expressed in human cells, play a crucial role in cell bioenergetics by catalyzing ADP and ATP exchange across the mitochondrial inner membrane. In contrast to differentiated tissue cells, transformed cells, and their ρ0 derivatives, i.e. cells deprived of mitochondrial DNA, sustain a high rate of glycolysis. We compared the expression pattern of {ANT} isoforms in several transformed human cell lines at different stages of the cell cycle. The level of {ANT2} expression and glycolytic ATP production in these cell lines were in keeping with their metabolic background and their state of differentiation. The sensitivity of the mitochondrial inner membrane potential (Δψ) to several inhibitors of glycolysis and oxidative phosphorylation confirmed this relationship. We propose a new model for ATP uptake in cancer cells implicating the {ANT2} isoform, in conjunction with hexokinase II and the β subunit of mitochondrial ATP synthase, in the Δψ maintenance and in the aggressiveness of cancer cells.

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Authors and Affiliations

  1. Inserm, U694, Angers, F-49033, France

    Arnaud Chevrollier, Dominique Loiseau, Gilles Renier, Olivier Douay & Yves Malthièry

  2. Univ Angers, Angers, F-49035, France

    Arnaud Chevrollier, Dominique Loiseau, Gilles Renier, Olivier Douay & Yves Malthièry

  3. CHRU Angers, Service d' Immunologie, Angers, F-49033, France

    Arnaud Chevrollier, Dominique Loiseau, Gilles Renier, Olivier Douay & Yves Malthièry

  4. INRA, U1019, Clermont-Ferrand, F-63009, France

    Béatrice Chabi

  5. Univ Auvergne, Clermont-Ferrand, F-63001, France

    Béatrice Chabi & Georges Stepien

  6. Inserm, U484, Clermont-Ferrand, F-63005, France

    Georges Stepien

Authors
  1. Arnaud Chevrollier
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  2. Dominique Loiseau
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  3. Béatrice Chabi
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  4. Gilles Renier
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  5. Olivier Douay
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  6. Yves Malthièry
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  7. Georges Stepien
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Correspondence to Georges Stepien.

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Chevrollier, A., Loiseau, D., Chabi, B. et al. ANT2 Isoform Required for Cancer Cell Glycolysis. J Bioenerg Biomembr 37, 307–317 (2005). https://doi.org/10.1007/s10863-005-8642-5

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  • DOI: https://doi.org/10.1007/s10863-005-8642-5

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