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CD8+ T Cells in Facioscapulohumeral Muscular Dystrophy Patients with Inflammatory Features at Muscle MRI

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Abstract

Facioscapulohumeral muscular dystrophy (FSHD) is an inherited disease, and although strongly suggested, a contribution of inflammation to its pathogenesis has never been demonstrated. In FSHD patients, we found by immunohistochemistry inflammatory infiltrates mainly composed by CD8+ T cells in muscles showing hyperintensity features on T2-weighted short tau inversion recovery magnetic resonance imaging (T2-STIR-MRI) sequences. Therefore, we evaluated the presence of circulating activated immune cells and the production of cytokines in patients with or without muscles showing hyperintensity features on T2-STIR-MRI sequences and from controls. FSHD patients displaying hyperintensity features in one or more muscles showed higher CD8+pSTAT1+, CD8+T-bet+ T cells and CD14+pSTAT1+, CD14+T-bet+ cells percentages and IL12p40, IFNγ and TNFα levels than patients without muscles displaying hyperintense features and controls. Moreover, the percentages of CD8+pSTAT1+, CD8+T-bet+ and CD14+pSTAT1+ cells correlated with the proportion of muscles displaying hyperintensity features at T2-STIR sequences. These data indicate that circulating activated immune cells, mainly CD8+ T cells, may favour FSHD progression by promoting active phases of muscle inflammation.

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Acknowledgements

We thank Manuela Papacci and Teresa Maresca for technical assistance.

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Authors and Affiliations

  1. Institute of Neurology, Department of Neurosciences, Catholic University of Rome, largo Gemelli 8, 00168, Rome, Italy

    Giovanni Frisullo, Roberto Frusciante, Viviana Nociti, Giorgio Tasca, Rosaria Renna, Raffaele Iorio, Agata Katia Patanella, Elisabetta Iannaccone, Alessandro Marti, Monica Rossi, Assunta Bianco, Mauro Monforte, Pietro Attilio Tonali, Massimiliano Mirabella, Anna Paola Batocchi & Enzo Ricci

  2. Fondazione Don Carlo Gnocchi, Via Capecelatro 66, 20148, Milan, Italy

    Viviana Nociti, Agata Katia Patanella, Monica Rossi, Pietro Attilio Tonali, Massimiliano Mirabella & Enzo Ricci

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Correspondence to Anna Paola Batocchi.

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Frisullo, G., Frusciante, R., Nociti, V. et al. CD8+ T Cells in Facioscapulohumeral Muscular Dystrophy Patients with Inflammatory Features at Muscle MRI. J Clin Immunol 31, 155–166 (2011). https://doi.org/10.1007/s10875-010-9474-6

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  • DOI: https://doi.org/10.1007/s10875-010-9474-6

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