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Ganoderma lucidum polysaccharide peptide reduced the production of proinflammatory cytokines in activated rheumatoid synovial fibroblast

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Abstract

The aim of the current study was to elucidate the potential therapeutic effect of Ganoderma lucidum polysaccharide peptide (GL-PP) in rheumatoid arthritis (RA). The effects of GL-PP on cell proliferation and cytokine production were studied in RA synovial fibroblasts (RASF). GL-PP significantly inhibited the proliferation of RASF. Following the incubation with GL-PP, production of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 in RASF were significantly increased as expressed as percentage change from basal values. However, the actual effects were minimal due to the low basal values. When RASF were activated by IL-1β or lipopolysaccharides, IL-8 and MCP-1 production increased many folds. GL-PP significantly suppressed their productions. The inhibitory effects of GL-PP on cytokine production in RASF were at least in part, by inhibiting the nuclear factor-kappa B (NF-κB) transcription pathway. Our results demonstrated that GL-PP had the unique ability to modulate cytokine production in RASF and warrants further investigation into its mechanism of action.

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Acknowledgment

This study was supported by University Grants Council of Hong Kong.

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Authors and Affiliations

  1. Department of Pharmacology, University of Hong Kong, Hong Kong SAR, Hong Kong

    Y. W. Ho & R. Y. K. Man

  2. Department of Medicine, University of Hong Kong, Room 807 Administration Block, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong SAR, Hong Kong

    J. S. L. Yeung & C. S. Lau

  3. Department of Orthopaedics and Traumatology, University of Hong Kong, Hong Kong SAR, Hong Kong

    P. K. Y. Chiu & W. M. Tang

  4. Department of Pharmacology, Peking University Health Science Center, School of Basic Medical Sciences, Beijing, China

    Z. B. Lin

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  1. Y. W. Ho
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  3. P. K. Y. Chiu
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  4. W. M. Tang
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  7. C. S. Lau
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Correspondence to C. S. Lau.

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Ho, Y.W., Yeung, J.S.L., Chiu, P.K.Y. et al. Ganoderma lucidum polysaccharide peptide reduced the production of proinflammatory cytokines in activated rheumatoid synovial fibroblast. Mol Cell Biochem 301, 173–179 (2007). https://doi.org/10.1007/s11010-006-9409-y

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  • DOI: https://doi.org/10.1007/s11010-006-9409-y

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