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Proteomic studies of rat tibialis anterior muscle during postnatal growth and development

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Abstract

In this study, a proteomic analysis consisting of two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry was accomplished to investigate the complex protein expression patterns in rat tibialis anterior muscle during postnatal 3-month period. We determined the time-dependent expression alterations of 107 protein spots, among which 53 protein spots were identified. These identified proteins included skeletal contractile proteins, metabolic enzymes, chaperone, intermediate filament, and signal transduction proteins. The time-dependent expression of three proteins, such as Mylpf, desmin, and RKIP, was confirmed by Western blot analysis and immunohistochemistry. The functional implication of these expression changes was also discussed. We further analyzed the linkage and interactions among the differentially expressed proteins (MAPK1, RKIP, AHSG, etc.). Collectively, the results might add to the understanding of the molecular mechanisms regulating postnatal growth and development of rat tibialis anterior muscle.

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Abbreviations

TA:

Tibialis anterior muscles

2-DE:

Two-dimensional gel electrophoresis

MALDI-TOF/TOF MS:

Matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry

ASB-14:

Amidosulfobetaine-14

CHAPS:

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

DTT:

Dithiothreitol

EDTA:

Ethylene diamine tetraacetic acid

PMSF:

Phenylmethyl sulfonylfluoride

PVDF:

Polyvinylidene difluoride

RKIP:

Raf kinase inhibitor protein

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

TBP:

Tri-butyl-phosphate

TBST:

Tris buffered saline-Tween-20

TFA:

Trifluoroacetic acid

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Acknowledgments

This study was supported by Hi-Tech Research and Development Program of China (863 Program, Grant No. 2006AA02A128), National Natural Science Foundation of China (Grant No.30670667) and University of Jiangsu Province Key Technological Research Project of China (Grant No. 05KJA31010). We thank professor Jie Liu for the assistance in manuscript preparation.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Hualin Sun, Ting Zhu, Fei Ding, Nan Hu & Xiaosong Gu

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  1. Hualin Sun
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  2. Ting Zhu
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  3. Fei Ding
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  4. Nan Hu
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  5. Xiaosong Gu
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Correspondence to Xiaosong Gu.

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Hualin Sun and Ting Zhu contributed equally to this work.

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Sun, H., Zhu, T., Ding, F. et al. Proteomic studies of rat tibialis anterior muscle during postnatal growth and development. Mol Cell Biochem 332, 161–171 (2009). https://doi.org/10.1007/s11010-009-0186-2

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  • DOI: https://doi.org/10.1007/s11010-009-0186-2

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