Skip to main content
SpringerLink
Log in

Endopolygalacturonase: a Candidate Gene for Freestone and Melting Fleshin Peach

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Peach fruit are handled, processed, and marketed according to their stone adhesion and fruit softening type. Uncertainty exists over whether these simply inherited traits are controlled by two linked loci, Freestone (F) and Melting flesh (M) or one multi-allelic locus, and whether M is controlled by the cell wall degrading enzyme, endopolygalacturonase. From morphological and molecular analysis of two related segregating populations of peach, we conclude that a single locus containing at least one gene for endopolygalacturonase, controls both F and M with at least three effective alleles. A simple diagnostic PCR test is now available for the three major phenotypes of freestone melting flesh (FMF), clingstone melting flesh (CMF), and clingstone non-melting flesh (CNMF).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • M.J. Aranzana A. Pineda P. Cosson E. Dirlewanger J. Ascasibar G. Cipriani C.D. Ryder R. Testolin A. Abbott G.J. King A.F. Iezzoni P. Arus (2003) ArticleTitleA set of simple-sequence repeat (SSR) markers covering the Prunus genome Theor. Appl. Genet 106 819–825 Occurrence Handle12647055

    PubMed  Google Scholar 

  • J.S. Bailey A.P. French (1933) ArticleTitleThe inheritance of certain characteristics in the peach Proc. Amer. Soc. Hortic. Sci 29 127–130

    Google Scholar 

  • Bailey J.S. and French A.P. 1949. The inheritance of certain fruit and foliage characteristics in the peach. Mass. Agr. Expt. Sta. Bul. 452.

  • D.A. Brummell M.H. Harpster (2001) ArticleTitleCell wall metabolism in fruit softening and quality and its manipulation in transgenic plants Plant Mol. Biol 47 311–340 Occurrence Handle10.1023/A:1010656104304 Occurrence Handle11554479

    Article  PubMed  Google Scholar 

  • A.M. Callahan R. Scorza C. Bassett M. Nickerson F.B. Abeles (2004) ArticleTitleDeletions in an endopolygalacturonase gene cluster correlate with non-melting flesh texture in peach Func. Plant Biol 31 159–168 Occurrence Handle10.1071/FP03131

    Article  Google Scholar 

  • CUGI. 2003. Clemson University Genomics Institute Peach EST Project. URL http://www.enome.clemson.edu/projects/peach/est/.

  • M.T. Dettori R. Quarta I. Verde (2001) ArticleTitleA peach linkage map integrating RFLPs, SSRs, RAPDs, and morphological markers Genome 44 783–790 Occurrence Handle10.1139/gen-44-5-783 Occurrence Handle11681601

    Article  PubMed  Google Scholar 

  • E. Dirlewanger P. Cosson M. Tavaud M.J. Aranzana C. Poizat A. Zanetto P. Arus F. Laigret (2002) ArticleTitleDevelopment of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.) Theor. Appl. Genet 105 127–138 Occurrence Handle10.1007/s00122-002-0867-7 Occurrence Handle12582570

    Article  PubMed  Google Scholar 

  • C. Etienne C. Rothan A. Moing C. Plomion C. Bodenes L. Svanella-Dumas P. Cosson V. Pronier R. Monet E. Dirlewanger (2002) ArticleTitleCandidate genes and QTLs for sugar and organic acid content in peach [ Prunus persica(L.) Batsch] Theor. Appl. Genet 105 145–159 Occurrence Handle10.1007/s00122-001-0841-9 Occurrence Handle12582572

    Article  PubMed  Google Scholar 

  • M.R. Foolad S. Arulsekar V. Becerra F.A. Bliss (1995) ArticleTitleA genetic map of Prunus based on an interspecific cross between peach and almond Theor. Appl. Genet 91 262–269 Occurrence Handle10.1007/BF00220887

    Article  Google Scholar 

  • K.A. Hadfield A.B. Bennett (1998) ArticleTitlePolygalacturonases: many genes in search of a function Plant Physiol 117 337–343 Occurrence Handle10.1104/pp.117.2.337 Occurrence Handle9625687

    Article  PubMed  Google Scholar 

  • K.A. Hadfield J.K.C. Rose D.S. Yaver R.M. Berka A.B. Bennett (1998) ArticleTitlePolygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly Plant Physiol 117 363–373 Occurrence Handle10.1104/pp.117.2.363 Occurrence Handle9625689

    Article  PubMed  Google Scholar 

  • T. Hayashi T. Yamamoto (2002) ArticleTitleGenome research on peach and pear J. Plant Biotech 42 45–52

    Google Scholar 

  • K. Hiwasa Y. Kinugasa S. Amano A. Hashimoto R. Nakano A. Inaba Y. Kubo (2003) ArticleTitleEthylene is required for both the initiation and progression of softening in pear (Pyrus communis L.) fruit J. Exp. Bot 54 771–779 Occurrence Handle10.1093/jxb/erg073 Occurrence Handle12554720

    Article  PubMed  Google Scholar 

  • E. Lee J. Speirs J. Gray C.J. Brady (1990) ArticleTitleHomologies to the tomato endopolygalacturonase gene in the peach genome Plant Cell Environ 13 513–521

    Google Scholar 

  • D.R. Lester W.B. Sherman B.J. Atwell (1996) ArticleTitleEndopolygalacturonase and the melting flesh (M) locus in peach J. Amer. Soc. Hort. Sci 121 231–235

    Google Scholar 

  • D.R. Lester J. Speirs G. Orr C.J. Brady (1994) ArticleTitlePeach (Prunus persica) endopolygalacturonase cDNA isolation and mRNA analysis in melting and nonmelting peach cultivars Plant Physiol 105 225–231 Occurrence Handle10.1104/pp.105.1.225 Occurrence Handle8029352

    Article  PubMed  Google Scholar 

  • R. Monet (1989) ArticleTitlePeach genetics: pastpresent and future Acta Hort 254 49–53

    Google Scholar 

  • Okie W.R. 1998. Handbook of peach and nectarine varieties: Performance in the southeastern United States and index of names. US Department of Agriculture, Agriculture Handbook No. 714.

  • G. Orr C. Brady (1993) ArticleTitleRelationship of endopolygalacturonase activity to fruit softening in a freestone peach Postharvest Biol. Biotech 3 121–130 Occurrence Handle10.1016/0925-5214(93)90004-M

    Article  Google Scholar 

  • R. Pressey J.K. Avants (1978) ArticleTitleDifference in polygalacturonase composition of clingstone and freestone peaches J. Food Sci 43 1415–1423

    Google Scholar 

  • R. Pressey D.M. Hinton J.K. Avants (1971) ArticleTitleDevelopment of polygalacturonase activity and solubilization of pectin in peaches during ripening J. Food Sci 36 1070–1073

    Google Scholar 

  • G.U. Rao I. Paran (2003) ArticleTitlePolygalacturonase: a candidate gene for the soft flesh and deciduous fruit mutation in Capsicum Plant Mol. Biol 51 135–141 Occurrence Handle12602897

    PubMed  Google Scholar 

  • J. Redondo-Nevado E. Moyano N. Medino-Escobar J.L. Caballero J. Munoz-Blanco (2001) ArticleTitleA fruit-specific and developmentally regulated endopolygalacturonase gene from strawberry (Fragaria ananassa cv. Chandler) J. Exp. Bot 52 1941–1945 Occurrence Handle10.1093/jexbot/52.362.1941 Occurrence Handle11520883

    Article  PubMed  Google Scholar 

  • Rozen S. and Skaletsky H.J. 1998. Primer3. Code available at http://www-genome.wi.mit.edu/genome_software/other/primer3. html.

  • L. Trainotti D. Zanin G. Casadoro (2003) ArticleTitleA cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches J. Exp. Bot 54 1821–1832 Occurrence Handle10.1093/jxb/erg198 Occurrence Handle12815031

    Article  PubMed  Google Scholar 

  • C.R. Van Der Heyden P. Holford G.D. Richards (1997) ArticleTitleA new source of peach germplasm containing semi-freestone non-melting flesh types HortScience 32 288–289

    Google Scholar 

  • J. Waldron C.P. Peace I.R. Searle A. Furtado N. Wade I. Findlay M.W. Graham B.J. Carroll (2002) ArticleTitleRandomly amplified DNA fingerprinting (RAF): a culmination of DNA marker technologies based on arbitrarily-primed PCR amplification J. Biomed. Biotech 2 141–150 Occurrence Handle10.1155/S1110724302206026

    Article  Google Scholar 

  • K.R. Weaver G. Caetano-Anolles P.M. Gresshoff L.M. Callahan (1994) ArticleTitleIsolation and cloning of DNA amplification products from silver-stained polyacrylamide gels Biotechniques 16 226–227 Occurrence Handle7514004

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kearney Agricultural Center, Department of Pomology, University of California, Davis, 9240 S Riverbend Ave, Parlier, CA, 93648, USA

    C.P. Peace & C.H. Crisosto

  2. Department of Pomology, University of California, Davis, 1047 Wickson Hall Davis, CA, 95616, USA

    T.M. Gradziel

Authors
  1. C.P. Peace
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C.H. Crisosto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T.M. Gradziel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C.P. Peace.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peace, C., Crisosto, C. & Gradziel, T. Endopolygalacturonase: a Candidate Gene for Freestone and Melting Fleshin Peach. Mol Breeding 16, 21–31 (2005). https://doi.org/10.1007/s11032-005-0828-3

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-005-0828-3

Keywords

Search

Navigation