Abstract
Sequence-characterized amplified regions markers (SCARs) were developed from six randomly amplified polymorphic DNA (RAPD) markers linked to the major QTL region for powdery mildew (Uncinula necator) resistance in a test population derived from the cross of grapevine cultivars “Regent” (resistant) × “Lemberger”(susceptible). RAPD products were cloned and sequenced. Primer pairs with at least 21 nucleotides primer length were designed. All pairs were tested in the F1 progeny of “Regent” × “Lemberger”. The SCAR primers resulted in the amplification of specific bands of expected sizes and were tested in additional genetic resources of resistant and susceptible germplasm. All SCAR primer pairs resulted in the amplification of specific fragments. Two of the SCAR markers named ScORA7-760 and ScORN3-R produced amplification products predominantly in resistant individuals and were found to correlate to disease resistance. ScORA7-760, in particular, is suitable for marker-assisted selection for powdery mildew resistance and to facilitate pyramiding powdery mildew resistance genes from various sources.
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Acknowledgments
This work has been supported as a Ph.D. fellowship for M.A. provided by the University of Ankara, Turkey, by the German Science Foundation (DFG, Deutsche Forschungsgemeinschaft), the German Academic Exchange Servie (DAAD, Deutscher Akademischer Austauschdienst), the Forschungsring des Deutschen Weinbaues (FDW) bei der DLG, and the Friends and Supporters of the Institute for Grapevine Breeding Geilweilerhof (Freunde und Förderer des Instituts für Rebenzüchtung Geilweilerhof e.V.) as well as the basic funding through the German Federal Ministry for Nutrition, Agriculture and Consumer Protection (BMELV, Bundesministerium für Ernährung, Landwirtschaft und Verbraucherschutz). We thank Heike Bennek, Claudia Welsch and Ulla Scherer for expert technical assistance.
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Akkurt, M., Welter, L., Maul, E. et al. Development of SCAR markers linked to powdery mildew (Uncinula necator) resistance in grapevine (Vitis vinifera L. and Vitis sp.). Mol Breeding 19, 103–111 (2007). https://doi.org/10.1007/s11032-006-9047-9
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DOI: https://doi.org/10.1007/s11032-006-9047-9