Skip to main content

Advertisement

Log in

Mapping and validation of a major QTL for yellow pigment content on 7AL in durum wheat (Triticum turgidum L. ssp. durum)

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Yellow pigment content in durum wheat (Triticum turgidum L. ssp. durum) is an important criterion for both pasta bright yellow color and human health because of antioxidant properties of carotenoids involved in this pigmentation. In the present study, QTLs for yellow pigment content in durum wheat were mapped in a population of 140 RILs developed from a intraspecific cross between a released variety (PDW 233) and a landrace (Bhalegaon 4). This trait was evaluated in one location for 3 years and in two more locations for one additional year (five different year × location combinations further called “environments”). Yellow pigment content was highly heritable across the five different environments. Analysis of variance showed the significant effect of genotype, environment and genotype × environment interaction on the trait. Five different QTLs linked to yellow pigment content were identified on chromosome 1A, 3B, 5B, 7A and 7B across five different environments. The strongest one located on the distal part of the long arm of chromosome 7A, QYp.macs-7A, explained 55.22% of the variation in the trait, while, remaining four QTLs explained 5–8.75% of phenotypic variation in yellow pigment content. Marker analysis revealed significant association of one ISSR and one AFLP fragment with the trait. These two markers were linked to the major QTL QYp.macs-7A and were converted into SCAR markers. These SCAR markers were further validated on another population as well as 38 diverse genotypes so as to prove their potential in marker assisted selection. These markers will be very useful for the marker assisted breeding of durum wheat for higher yellow pigment content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Basten CJ, Weir BS, Zeng Z-B (1994) Zmap—a QTL cartographer. In: Smith C, Gavora JS, Benkel J, Chesnais B, Fairfull W, Gibson JP, Kennedy BW, Burnsid EB (eds) Proceedings of the 5th world congress on genetics applied to livestock production: computing strategies and software, vol 22. Organizing Committee, 5th World Congress on Genetics Applied to Livestock Production, Guelph, Ontario, pp 65–66

  • Carrera A, Echenique V, Zhang W, Helguera M, Manthey F, Schrager A, Picca A, Cervigni G, Dubcovsky J (2007) A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum). J Cereal Sci 45:67–77

    Article  CAS  Google Scholar 

  • Clarke FR, Clarke JM, McCaig TN, Knox RE, DePauw RM (2006) Inheritance of yellow pigment concentration in four durum wheat crosses. Can J Plant Sci 86:133–141

    Google Scholar 

  • Elouafi I, Nachit MM (2004) A genetic linkage map of the Durum × Triticum dicoccoides L backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theor Appl Genet 108:401–413

    Article  PubMed  CAS  Google Scholar 

  • Elouafi I, Nachit MM, Martin LM (2001) Identification of a microsatellite on chromosome 7B showing a strong linkage with yellow pigment in durum wheat (Triticum turgidum L. var. durum). Hereditas 135:255–261

    Article  PubMed  CAS  Google Scholar 

  • Eujayl I, Sorrells ME, Baum M, Wolters P, Powell W (2002) Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor Appl Genet 104:399–407

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Casal MN (2006) Carotenoids increase iron absorption from cereal-based food in the human. Nutrition Res 26:340–344

    Article  CAS  Google Scholar 

  • Hessler TG, Thomson MJ, Benscher D, Nachit MM, Sorrells ME (2002) Association of a lipoxygenase locus, Lpx-B1, with variation in lipoxygenase activity in durum wheat seeds. Crop Sci 42:1695–1700

    Article  CAS  Google Scholar 

  • Johnston RA, Quick JS, Hammond JJ (1983) Inheritance of semolina colour in six durum wheat crosses. Crop Sci 23:607–610

    Article  Google Scholar 

  • Joppa LR, Williams ND (1988) Genetics and breeding of durum wheat in the United States. In: Fabriani G, Lintas C, (eds) Durum wheat: chemistry and technology. American Association of Cereal Chemists, Minnesota, USA, pp 47–68

    Google Scholar 

  • Krinsky NI (1994) The biological properties of carotenoids. Pure Appl Chem 66:1003–1010

    Article  Google Scholar 

  • Lepage M, Sims RPA (1968) Carotenoids of wheat flour: their identification and composition. Cereal Chem 45:600–604

    CAS  Google Scholar 

  • Liu HZ, Anderson JA, Hu J, Friesen TL, Rasmussen JB, Faris JD (2005) A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor Appl Genet 111:782–794

    Article  PubMed  CAS  Google Scholar 

  • Mares DJ, Campbell AW (2001) Mapping components of flour and noodle colour in Australian wheat. Aust J Agric Res 52:1297–1309

    Article  CAS  Google Scholar 

  • Mares-Perlman JA, Millen AE, Ficek TL, Hakinson SE (2002) The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease. Overview symposium: can lutein protect against chronic disease? J Nutr 132:518S–524S

    PubMed  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Mortensen A, Skibsted LH (1997) Importance of carotenoid structure in radical-scavenging reactions. J Agric Food Chem 45:2970–2977

    Article  CAS  Google Scholar 

  • Moise AR, von Lintig J, Palczewski K (2005) Related enzymes solve evolutionarily recurrent problems in the metabolism of carotenoids. Trends Plant Sci 10:178–186

    Article  PubMed  CAS  Google Scholar 

  • Nagaoka T, Ogihara Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597–602

    Article  CAS  Google Scholar 

  • Parker GD, Chalmers KJ, Rathjen AJ, Langridge P (1998) Mapping loci associated with flour colour in wheat (Triticum aestivum L.). Theor Appl Genet 97:238–245

    Article  CAS  Google Scholar 

  • Parker GD, Langridge P (2000) Development of a STS marker linked to a major locus controlling flour colour in wheat (Triticum aestivum L.). Mol Breed 6:169–174

    Article  CAS  Google Scholar 

  • Patil RM, Oak MD, Tamhankar SA, Rao VS (2006) Identification of gluten protein subunits and their PCR amplified products related to sedimentation volume in durum wheat. J Plant Biochem Biotechnol 15:47–50

    CAS  Google Scholar 

  • Pozniak CJ, Knox RE, Clarke FR, Clarke JM (2007) Identification of QTL and association of a phytoene synthase gene with endosperm colour in durum wheat. Theor Appl Genet 114:525–537

    Article  PubMed  CAS  Google Scholar 

  • Prasad M, Kumar N, Kulwal PL, Röder MS, Balyan HS, Dhaliwal HS, Roy JK, Gupta PK (2003) QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor Appl Genet 106:659–667

    PubMed  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Roger SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76

    Article  Google Scholar 

  • Romer S, Fraser PD (2005) Recent advances in carotenoid biosynthesis, regulation and manipulation. Planta 221:305–308

    Article  PubMed  CAS  Google Scholar 

  • Santra M (2005) Study of inheritance and identification of molecular markers for β-carotene content in durum. Ph. D. Thesis, University of Pune, Pune, India

  • Santra M, Rao VS, Tamhankar SA (2003) Modification of AACC procedure for measuring β-carotene in early generation durum wheat. Cereal Chem 80:130–131

    Article  CAS  Google Scholar 

  • Santra M, Rao VS, Tamhankar SA (2006) Biochemical estimation of beta carotene in Indian durum varieties. Wheat Inf Serv 102:1–4 http://www.shigen.nig.ac.jp/ewis Cited 5 Sep 2006

  • Santra M, Santra DK, Rao VS, Taware SP, Tamhankar SA (2005) Inheritance of β-carotene concentration in durum wheat (Triticum turgidum L. ssp. durum). Euphytica 144:215–221

    Article  CAS  Google Scholar 

  • Sharp PJ, Johnston S, Brown G, McIntosh RA, Pallotta M, Carter M, Bariana HS, Khartkar S, Lagudah ES, Singh RP, Khairallah M, Potter R, Jones MGK (2001) Validation of molecular markers for wheat breeding. Aust J Agric Res 52:1357–1366

    Article  CAS  Google Scholar 

  • Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci 14:203–208

    Article  Google Scholar 

  • Torada A, Ikeguchi S, Koike M (2005) Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat. Euphytica 143:251–255

    Article  CAS  Google Scholar 

  • Troccoli A, Borrelli GM, De Vita P, Fares C, Di Fonzo N (2000) Durum wheat quality: a multidisciplinary concept. J Cereal Sci 32:99–113

    Article  Google Scholar 

  • Trono D, Pastore D, Di Fonzo N (1999) Carotenoid dependent inhibition of durum wheat lipoxygenase. J Cereal Sci 29:99–102

    Article  CAS  Google Scholar 

  • Wang S, Basten CJ, Zeng Z (2004) Windows QTL cartographer. V2.0 Program in statistical genetics, North Carolina State University, North Carolina. http://www.statgen.ncsu.edu/qtlcart/WQTLCart.htm

  • Yu J-K, Dake TM, Singh S, Benscher D, Li W, Gill B, Sorrells ME (2004) Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat. Genome 47:805–818

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful to Directorate of Wheat Research (DWR), Karnal, especially Dr. Ratan Tiwari and Indian Agricultural Research Institute (IARI), Regional Research Station, Indore, especially Dr. H. N. Pandey for providing facilities to conduct field trials during 2002–2003. Thanks are also due to Steven S Xu, USDA-ARS, Cereal Crop Research Unit, Northern Crop Science Laboratory, Fargo, ND, USA for providing the Langdon- dicoccoides disomic substitution lines.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Veliventi S. Rao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Patil, R.M., Oak, M.D., Tamhankar, S.A. et al. Mapping and validation of a major QTL for yellow pigment content on 7AL in durum wheat (Triticum turgidum L. ssp. durum). Mol Breeding 21, 485–496 (2008). https://doi.org/10.1007/s11032-007-9147-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-007-9147-1

Keywords

Navigation