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Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

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Abstract

An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

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Acknowledgements

The authors would like to acknowledge the excellent technical support from involved institutions, among them D. Cadier, P. Cormenier and C. Gibelin from INRA, Lusignan, France as well as Y. Häfele and L. Kren from ART, Zurich, Switzerland. Special thanks go to G. Dioniso for excellent support in bioinformatics. This project was kindly supported through the Directorate for Food, Fisheries and Agri Business (DFFE), Denmark.

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Centre Flakkebjerg, University of Aarhus, Forsøgsvej 1, Slagelse, 4200, Denmark

    Bruno Studer, Torben Asp, Ursula Frei, Stephan Hentrup & Thomas Lübberstedt

  2. Crops Research Centre Oak Park, TEAGASC, Carlow, Ireland

    Helena Meally, Aurélie Guillard & Susanne Barth

  3. Plant Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Melle, Belgium

    Hilde Muylle & Isabel Roldán-Ruiz

  4. Unité de Génétique et d’Amélioration des Plantes Fourragères, Institut National de Recherche Agronomique (INRA), Lusignan, France

    Philippe Barre

  5. Plant Research International B.V. (PRI), Wageningen, The Netherlands

    Carole Koning-Boucoiran, Gerda Uenk-Stunnenberg & Oene Dolstra

  6. Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research (IGER), Plas Gogerddan, Aberystwyth, UK

    Leif Skøt, Kirsten P. Skøt, Lesley B. Turner & Mervyn O. Humphreys

  7. Agroscope Reckenholz-Tänikon Research Station (ART), Zurich, Switzerland

    Roland Kölliker

  8. DLF-TRIFOLIUM, Dansk Planteforædling, Store Heddinge, Denmark

    Niels Roulund & Klaus K. Nielsen

Authors
  1. Bruno Studer
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  2. Torben Asp
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  3. Ursula Frei
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  4. Stephan Hentrup
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  5. Helena Meally
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  8. Hilde Muylle
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  9. Isabel Roldán-Ruiz
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  10. Philippe Barre
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  11. Carole Koning-Boucoiran
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  12. Gerda Uenk-Stunnenberg
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  13. Oene Dolstra
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  14. Leif Skøt
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  15. Kirsten P. Skøt
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  16. Lesley B. Turner
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  17. Mervyn O. Humphreys
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  18. Roland Kölliker
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  19. Niels Roulund
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  20. Klaus K. Nielsen
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  21. Thomas Lübberstedt
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Corresponding author

Correspondence to Bruno Studer.

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Studer, B., Asp, T., Frei, U. et al. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Mol Breeding 21, 533–548 (2008). https://doi.org/10.1007/s11032-007-9148-0

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  • DOI: https://doi.org/10.1007/s11032-007-9148-0

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