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Molecular characterization and expression analysis of OsBISERK1, a gene encoding a leucine-rich repeat receptor-like kinase, during disease resistance responses in rice

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Abstract

A rice gene, OsBISERK1, encoding a protein belonging to SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) type of leucine-rich repeat receptor-like kinases (LRR-RLKs) was identified. The OsBISERK1 encodes a 624 aa protein with high level of identity to known plant SERKs. OsBISERK1 contains a hydrophobic signal peptide, a leucine zipper, and five leucine-rich repeat motifs in the extracellular domain; the cytoplasmic region carries a proline-rich region and a single transmembrane domain, as well as a conserved intracellular serine/threonine protein kinase domain. OsBISERK1 has a low level of basal expression in leaf tissue. However, expression of OsBISERK1 was induced by treatment with benzothiadiazole (BTH), which is capable of inducing disease resistance in rice, and also up-regulated after inoculation with Magnaporthe grisea in BTH-treated rice seedlings and during incompatible interaction between a blast-resistant rice genotype and M. grisea. The results suggest that OsBISERK1 may be involved in disease resistance responses in rice.

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Abbreviations

Avr:

Avirulence

BTH:

Benzothiadiazole

LRR:

Leucine-rich repeat

R:

Resistance

RLK:

Receptor-like kinase

SA:

Salicylic acid

SAR:

Systemic acquired resistance

SERK:

Somatic embryogenesis receptor kinase

Ser/Thr:

Serine/Threonine

References

  1. Martin GB, Bogdanove AJ, Sessa G (2003) Understanding the functions of plant disease resistance proteins. Annu Rev Plant Biol 54:23–61

    Article  PubMed  CAS  Google Scholar 

  2. Rathjen JP, Moffett P (2003) Early signal transduction events in specific plant disease resistance. Curr Opin Plant Biol 6:300–306

    Article  PubMed  CAS  Google Scholar 

  3. Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, Hunt MD (1996) Systemic acquired resistance. Plant Cell 8:1809–1819

    Article  PubMed  CAS  Google Scholar 

  4. Sticher L, Mauch-Mani B, Metraux JP (1997) Systemic acquired resistance. Annu Rev Phytopathol 35:235–270

    Article  PubMed  CAS  Google Scholar 

  5. Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815

    Article  Google Scholar 

  6. Morris ER, Walkery JC (2003) Receptor-like protein kinases: the keys to response. Curr Opin Plant Biol 6:339–342

    Article  PubMed  CAS  Google Scholar 

  7. Becraft PW (1998) Receptor kinases in plant development. Trends Plant Sci 3:384–388

    Article  Google Scholar 

  8. Carole LB, Michael LN, Reuben AC, Mangalathu SR (2000) Alternative transcript initiation and novel post-transcriptional processing of a leucine-rich repeat receptor-like protein kinase gene that responds to short-day photoperiodic floral induction in morning glory (Ipomoea nil). Plant Mol Biol 43:43–58

    Article  Google Scholar 

  9. Nishiguchi M, Yoshida K, Sumizono T, Tazaki K (2002) A receptor-like protein kinase with a lectin-like domain from lombardy poplar: gene expression in response to wounding and characterization of phosphorylation activity. Mol Genet Genomics 267:506–514

    Article  PubMed  CAS  Google Scholar 

  10. Hong SW, Jon JH, Kwak JM, Nam HG (1997) Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt, and cold treatments in Arabidopsis thaliana. Plant Physiol 113:1203–1212

    Article  PubMed  CAS  Google Scholar 

  11. Li J, Chory J (1997) A putative leucine-rich repeat receptor kinase involved in brassionsteroid signal transduction. Cell 90:929–938

    Article  PubMed  CAS  Google Scholar 

  12. Li J, Wen J, Lease KA, Doke JT, Tax FE, Walker JC (2002) BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell 110:213–222

    Article  PubMed  CAS  Google Scholar 

  13. Song WY, Wang GL, Chen LL, Kim HS, Pi LY, Holsten T, Gardner J, Wang B, Zhai WX, Zhu LH, Fauquet C, Ronald P (1995) A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270:1804–1806

    Article  PubMed  CAS  Google Scholar 

  14. Gomez-Gomez L, Boller T (2000) FLS2: A LRR receptor-like kinase involved in recognition of the flagellin elicitor in Arabidopsi. Mol Cell 5:1–20

    Article  Google Scholar 

  15. Silva N, Goring D (2002) The proline-rich, extensin-like receptor kinase-1 (PERK1) gene is rapidly induced by wounding. Plant Mol Biol 50:667–685

    Article  CAS  Google Scholar 

  16. Schmidt EDL, Guzzo F, Toonen MAJ, de Vries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124:2049–2062

    PubMed  CAS  Google Scholar 

  17. Hecht V, Vielle-Calzada JP, Hartog MV, Schmidt EDL, Boutilier K, Grossniklaus U, de Vries SC (2001) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol 127:803–816

    Article  PubMed  CAS  Google Scholar 

  18. Baudino S, Hansen S, Brettschneider R, Hecht VFG, Dresselhaus T, Lorz H, Dumas C, Rogowsky PM (2001) Molecular characterization of novel maize LRR receptor-like kinases, which belong to the SERK gene family. Planta 213:1–10

    Article  PubMed  CAS  Google Scholar 

  19. Ito Y, Takaya K, Kurata N (2005) Expression of SERK family receptor-like protein kinase genes in rice. Biochim Biophys Acta 1730:253–258

    PubMed  CAS  Google Scholar 

  20. Nolan KE, Irwanto RR, Rose RJ (2003) Auxin up-regulates MtSERK1 expression in both Medicago truncatula root-forming and embryogenic cultures. Plant Physiol 133:218–230

    Article  PubMed  CAS  Google Scholar 

  21. Albrecht C, Russinova E, Hecht V, Baaijens E, de Vries S. (2005) The Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES1 and 2 control male sporogenesis. Plant Cell 17:3337–3349

    Article  PubMed  CAS  Google Scholar 

  22. Hu H, Xiong L, Yang Y (2005) Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection. Planta 222:107–117

    Article  PubMed  CAS  Google Scholar 

  23. Luo H, Song F, Goodman RM, Zheng Z (2005) Up-regulation of OsBIHD1, a rice gene encoding BELL homeodomain transcriptional factor, in disease resistance responses. Plant Biol 7:459–468

    Article  PubMed  CAS  Google Scholar 

  24. Song F, Goodman RM (2002) OsBIMK1, a rice MAP kinase gene involved in disease resistance responses. Planta 215:997–1005

    Article  PubMed  CAS  Google Scholar 

  25. Gorlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel KH, Oostendorp M, Staub T, Ward E, Kessmann H, Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8:629–643

    Article  PubMed  CAS  Google Scholar 

  26. Ge X, Song F, Zheng Z (1999) Systemic acquired resistance to Magnaporthe grisea in rice induced by BTH. Acta Agri Zhejiangensis 11:311–314

    Google Scholar 

  27. Song F, Ge X, Zheng Z, Xie Y (2001) Benzothiadiazole induces systemic acquired resistance in rice against bacterial leaf blight. Chinese J Rice Sci 15:323–326

    Google Scholar 

  28. Zhang W, Ge X, Song F, Zheng Z (2004) Systemic acquired resistance of rice against sheath blight disease induced by benzothiadiazole. Acta Phytophyl Sin 30:171–176

    Google Scholar 

  29. Xiong L, Lee MW, Qi M, Yang Y (2001) Identification of defense-related rice genes by suppression subtractive hybridization and differential screening. Mol Plant-Microbe Interact 14:685–692

    Article  PubMed  CAS  Google Scholar 

  30. Cock JM, Vanoosthuyse V, Gaude T (2002) Receptor kinase signaling in plants and animals: distinct molecular systems with mechanistic similarities. Curr Opin Cell Biol 14:230–236

    Article  PubMed  CAS  Google Scholar 

  31. Lease K, Ingham E, Walker JC (1998) Challenges in understanding RLK function. Curr Opin Plant Biol 1:388–392

    Article  PubMed  CAS  Google Scholar 

  32. Kajava AV, Vassart G, Wodak SJ (1995) Modeling of the three-dimensional structure of proteins with the typical leucine-rich repeats. Structure 3:867–877

    Article  PubMed  CAS  Google Scholar 

  33. Kobe B, Deisenhofter J (1994) The leucine-rich repeat: a versatile binding motif. Trends Biochem Sci 19:415–421

    Article  PubMed  CAS  Google Scholar 

  34. Clark SE, Williams RW, Meyerowitz EM (1997) The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis. Cell 59:575–585

    Article  Google Scholar 

  35. Bauer Z, Gomez-Gomez L, Boller T, Felix G (2001) Sensitivity of different ecotypes and mutants of Arabidopsis thaliana toward the bacterial elicitor flagellin correlates with the presence of receptor-binding sites. J Biol Chem 276:45669–45676

    Article  PubMed  CAS  Google Scholar 

  36. Hajouj T, Michelis R, Gepstein S (2000) Cloning and characterization of a receptor-like protein kinase gene associated with senescence. Plant Physiol 124:1305–1314

    Article  PubMed  CAS  Google Scholar 

  37. Thomas C, Meyer D, Himber C, Steinmetz A (2004) Spatial expression of a sunflower SERK gene during induction of somatic embryogenesis and shoot organogenesis. Plant Physiol Biochem 42:35–42

    Article  PubMed  CAS  Google Scholar 

  38. Luo H, Song F, Zheng Z (2005) Overexpression in transgenic tobacco reveals different roles for the rice homeodomain gene OsBIHD1 in biotic and abiotic stress responses. J Exp Bot 56:2673–2682

    Article  PubMed  CAS  Google Scholar 

  39. Song F, Goodman RM (2002) Molecular cloning and characterization of a rice phosphoinositide-specific phospholipase C gene, OsPI-PLC1 that is activated in systemic acquired resistance. Physiol Mol Plant Pathol 61:31–40

    CAS  Google Scholar 

  40. Song D, Chen J, Song F, Zheng Z (2006) A novel rice MAPK gene, OsBIMK2, is involved in disease-resistance responses. Plant Biol 8:587–596

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (grant no. 30170598), the Fund for the New Century Talent Program from MOE of China, and the Excellent Young Teachers Program of MOE to FMS. We thank Dr. Zuhua He (Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Science) for the rice near-isogenic lines H8R and H8S, and Mr. Rongyao Chai (Zhejiang Academy of Agricultural Science) for the Magnaporthe grisea isolate 85-14B1.

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310029, P.R. China

    Donghui Song, Guojun Li, Fengming Song & Zhong Zheng

  2. Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang, 310029, P.R. China

    Fengming Song

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  1. Donghui Song
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  2. Guojun Li
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  3. Fengming Song
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  4. Zhong Zheng
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Correspondence to Fengming Song.

Additional information

The nucleotide sequence of OsBISERK1 has been deposited in GenBank under accession number AY463361.

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Song, D., Li, G., Song, F. et al. Molecular characterization and expression analysis of OsBISERK1, a gene encoding a leucine-rich repeat receptor-like kinase, during disease resistance responses in rice. Mol Biol Rep 35, 275–283 (2008). https://doi.org/10.1007/s11033-007-9080-8

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  • DOI: https://doi.org/10.1007/s11033-007-9080-8

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