Abstract
Octanoylation of the gastric peptide ghrelin produces an active isoform that regulates appetite and other metabolic functions. Acylated ghrelin is present in the gastrointestinal tract suggesting that octanoylation may occur in these tissues and thereby affect the acylated ghrelin in the systemic circulation. In this study blood samples were collected simultaneously from portal, arterial, peripheral venous and central venous compartments from patients undergoing laparotomy. ELISA and high sensitivity Bioplex was used to measure the concentration of acylated and des acyl ghrelin. We found median (95% confidence interval (CI)) plasma acylated ghrelin (pg/ml) was 35.8 (30.0–59.6) in the portal compartment compared to 51.5 (37.6–74.8; P < 0.05, n = 11) in the arterial, 39.3 (33.3–56.3) in the portal compartment compared to 55.0 (48.5–77.0; P < 0.001, n = 12) in the peripheral venous and 36.0 (33.1–57.4) in the portal compartment compared to 48.9 (43.3–65.6; P < 0.01, n = 15) in the central venous compartment. Median (95% CI) plasma des acyl ghrelin levels (pg/ml) was 173 (125–220) in the portal compartment compared to 136 (99.3–125; P < 0.001, n = 14)in the arterial, 186 (136–233) in the portal compartment compared to 149 (111–190; P < 0.01, n = 15) in the peripheral venous and 171 (140–208) in the portal compartment compared to 152 (119–175; P < 0.01, n = 15) the central venous compartment. We conclude that plasma acylated ghrelin concentration was significantly lower in portal compared with the systemic compartments whilst plasma des acyl ghrelin was significantly higher in portal compared with systemic compartments. These findings suggest that the liver could be involved in the regulation of circulating ghrelin.
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References
Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo M, Kangawa K (1999) Ghrelin is a growth-hormone releasing acylated peptide from stomach. Nature 402:656–660
Wren AM, Seal LJ, Cohen MA, Brynes AE, Frost GS, Murphy KG, Dhillo WS, Ghatei MA, Bloom SR (2001) Ghrelin enhances appetite and increases food intake in humans. J Clin Endocrinol Metab 86:5992–5995
Cummings DE, Overduin J (2007) Gastrointestinal regulation of food intake. J Clin Investig 117:13–23
Inhoff T, Mönnikes H, Noetzel S, Stengel A, Goebel M, Dinh QT, Riedl A, Bannert N, Wisser AS, Wiedenmann B, Klapp BF, Taché Y, Kobelt P (2008) Desacyl ghrelin inhibits the orexigenic effect of peripherally injected ghrelin in rats. Peptides 29:2159–2168
Gardiner J, Bloom S (2008) Ghrelin gets its GOAT. Cell Metab 7(3):193–194
Mathur SN, Simon I, Lokesh BR, Spector AA (1983) Phospholipid fatty acid modification of rat liver microsomes affects acylcoenzyme A:cholesterol acyltransferase activity. Biochim Biophys Acta 751(3):401–411
Pellicoro A, van den Heuvel FA, Geuken M, Moshage H, Jansen PL, Faber KN (2007) Human and rat bile acid-CoA:amino acid N-acyltransferase are liver-specific peroxisomal enzymes: implications for intracellular bile salt transport. Hepatology 45(2):340–348
Gauna C, Uitterlinden P, Kramer P, Kiewiet RM, Janssen JA, Delhanty PJ, van Aken MO, Ghigo E, Hofland LJ, Themmen AP, van der Lely AJ (2007) Intravenous glucose administration in fasting rats has differential effects on acylated and unacylated ghrelin in the portal and systemic circulation: a comparison between portal and peripheral concentrations in anesthetized rats. Endocrinology 148(11):5278–5287
Xu Q, Bain H, Han N, Hou R, Zhang Z, Zhu M (2009) cDNA cloning and expression of ghrelin in gain panda (Ailuropoda melanoleuca). Mol Biol Rep. doi:10.1007/s11033-009-9850-6
Yang J, Brown MS, Liang G, Grishin NV, Goldstein JL (2008) Identification of the acyltransferase that octanoylates ghrelin, an appetite-stimulating peptide hormone. Cell 132(3):387–396
Date Y, Kojima M, Hosoda H, Sawaguchi A, Mondal MS, Suganuma T, Matsukura S, Kangawa K, Nakazato M (2000) Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology 141(11):4255–4261
Gutierrez JA, Solenberg PJ, Perkins DR, Willency JA, Knierman MD, Jin Z, Witcher DR, Luo S, Onyia JE, Hale JE (2008) Ghrelin octanoylation mediated by an orphan lipid transferase. Proc Natl Acad Sci USA 105(17):6213–6214
Peracchi M, Bardella MT, Caprioli F, Massironi S, Conte D, Valenti L, Ronchi C, Beck-Peccoz P, Arosio M, Piodi L (2006) Circulating ghrelin levels in patients with inflammatory bowel disease. Gut 55:432–433
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Goodyear, S., Arasaradnam, R.P., Quraishi, N. et al. Acylated and des acyl ghrelin in human portal and systemic circulations. Mol Biol Rep 37, 3697–3701 (2010). https://doi.org/10.1007/s11033-010-0022-5
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DOI: https://doi.org/10.1007/s11033-010-0022-5