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S100A4 mediated cell invasion and metastasis of esophageal squamous cell carcinoma via the regulation of MMP-2 and E-cadherin activity

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Abstract

It is well documented that S100A4 is upregulated in a large amount of invasive tumors and plays a pivotal role in tumor invasion and metastasis. However, the precise role and mechanism S100A4 exerts in the invasion and metastasis of esophageal squamous cell carcinoma (ESCC) have not been fully elucidated to date. Our data demonstrated that S100A4 was overexpressed in human ESCC tissues, especially in ESCC with poor differentiation, deep invasion and lymph node metastasis. Subsequently, the knockdown of S100A4 by RNAi in ESCC cell line (EC-1) could reduce cell invasion, metastasis and proliferation ability in vitro. Most importantly, S100A4 regulated MMP-2 positively and E-cadherin negatively in vivo and in vitro to some extent. Our results suggest that S100A4 is an important factor in the invasion, metastasis and proliferation of ESCC and may control invasion and metastasis at least in part through the regulation of MMP-2 and E-cadherin activity. S100A4 may serve as a biomarker for progression of ESCC and a potential molecular target for biotherapy of ESCC.

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Acknowledgments

We thank Prof. Cao (the University of Hong Kong) for providing the EC-1 cell line.

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Authors and Affiliations

  1. Department of Pathology, The First Affiliated Hospital of Zhengzhou University, 40 Daxue Road, Zhengzhou, Henan Province, 450052, People’s Republic of China

    Hong-Yan Zhang, Feng Wang & Shan-Shan Li

  2. Department of Neurology, The People’s Hospital of Jiaozuo City, Jiaozuo, Henan Province, 454002, People’s Republic of China

    Xian-Zhao Zheng

  3. Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, 450052, People’s Republic of China

    Xin-Hua Wang

  4. Department of Microbiology and Immunology, College of Basic Medicine of Zhengzhou University, Zhengzhou, Henan Province, 450001, People’s Republic of China

    Xiao-Yan Xuan

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  1. Hong-Yan Zhang
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Correspondence to Shan-Shan Li.

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Zhang, HY., Zheng, XZ., Wang, XH. et al. S100A4 mediated cell invasion and metastasis of esophageal squamous cell carcinoma via the regulation of MMP-2 and E-cadherin activity. Mol Biol Rep 39, 199–208 (2012). https://doi.org/10.1007/s11033-011-0726-1

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  • DOI: https://doi.org/10.1007/s11033-011-0726-1

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