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The 73 kD Subunit of the cleavage and polyadenylation specificity factor (CPSF) complex affects reproductive development in Arabidopsis

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Abstract

The cleavage and polyadenylation specificity factor (CPSF) is an important multi-subunit component of the mRNA 3′-end processing apparatus in eukaryotes. The Arabidopsis genome contains five genes encoding CPSF homologues (AtCPSF160, AtCPSF100, AtCPSF73-I, AtCPSF73-II and AtCPSF30). These CPSF homologues interact with each other in a way that is analogous to the mammalian CPSF complex or their yeast counterparts, and also interact with the Arabidopsis poly(A) polymerase (PAP). There are two CPSF73 like proteins (AtCPSF73-I and AtCPSF73-II) that share homology with the 73 kD subunit of the mammalian CPSF complex. AtCPSF73-I appears to correspond to the functionally characterized mammalian CPSF73 and its yeast counterpart. AtCPSF73-II was identified as a novel protein with uncharacterized protein homologues in other multicellular organisms, but not in yeast. Both of the AtCPSF73 proteins are targeted in the nucleus and were found to interact with AtCPSF100. They are also essential since knockout or knockdown mutants are lethal. In addition, the expression level of AtCPSF73-I is critical for Arabidopsis development because overexpression of AtCPSF73-I is lethal. Interestingly, transgenic plants carrying an additional copy of the AtCPSF73-I gene, that is, the full-length cDNA under the control of its native promoter, appeared normal but were male sterile due to delayed anther dehiscence. In contrast, we previously demonstrated that a mutation in the AtCPSF73-II gene was detrimental to the genetic transmission of female gametes. Thus, two 73 kD subunits of the AtCPSF complex appear to have special functions during flower development. The important roles of mRNA 3′-end processing machinery in modulating plant development are discussed.

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Abbreviations

AD:

activation domain

BD:

DNA binding domain

CF:

cleavage factor

CPSF:

cleavage and polyadenylation specificity factor

CstF:

cleavage stimulatory factor

CTD:

C-terminal domain

DEX:

dexamethasone

GFP:

green fluorescence protein

GST:

glutathione transferase

GUS:

β-glucuronidase

PAP:

poly(A) polymerase

RNAi:

RNA interference

RNAP II:

RNA polymerase II

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Authors and Affiliations

  1. Department of Botany, Miami University, Oxford, OH, 45056, USA

    Ruqiang Xu, Hongwei Zhao, George Carberry & Qingshun Quinn Li

  2. Department of Microbiology, Miami University, Oxford, OH, 45056, USA

    Xiaowen Cheng

  3. USDA–ARS Forage-Animal Production Research Unit, Lexington, KY40546, USA

    Randy D. Dinkins

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  1. Ruqiang Xu
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  2. Hongwei Zhao
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  3. Randy D. Dinkins
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  4. Xiaowen Cheng
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  5. George Carberry
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  6. Qingshun Quinn Li
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Correspondence to Qingshun Quinn Li.

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Gene accession numbers associated with this paper: AY140902, AY140900, AY168923, AY140901

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Xu, R., Zhao, H., Dinkins, R.D. et al. The 73 kD Subunit of the cleavage and polyadenylation specificity factor (CPSF) complex affects reproductive development in Arabidopsis . Plant Mol Biol 61, 799–815 (2006). https://doi.org/10.1007/s11103-006-0051-6

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