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Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size

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Abstract

The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher 3H-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.

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Abbreviations

APC:

Anaphase promoting complex

CaMV:

Cauliflower mosaic virus

NOS:

Nopaline sintase terminator

qPCR:

Quantitative polymerase chain reaction

SAM:

Shoot apical meristem

SDS:

Sodium dodecyl sulfate

TPR:

Tetratricopeptide repeat

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Acknowledgments

We are grateful to Ben Scheres for providing anti-AtCDC27a antibody. We are also thankful to Cropdesign (Belgium) for technical training. C.A.R is indebted to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), M.F.L. and N·B.E are indebted to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ) and BELSPO (Belgian Science Policy), for graduate and posdoctoral fellowships. P·C.G.F and A.S·H receive support from CNPq research grants.

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Authors and Affiliations

  1. Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brazil

    Cristian Antonio Rojas, Nubia Barbosa Eloy, Marcelo de Freitas Lima, Roberta Lopes Rodrigues, Adriana Silva Hemerly & Paulo Cavalcanti Gomes Ferreira

  2. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Diretoria de Pesquisa Científica, Rua Pacheco Leão 915, Rio de Janeiro, RJ, cep. 22460-030, Brazil

    Luciana Ozório Franco, Adriana Silva Hemerly & Paulo Cavalcanti Gomes Ferreira

  3. Department of Plant Systems Biology, VIB, Technologiepark 927, 9052, Ghent, Belgium

    Kristiina Himanen & Gerrit T. S. Beemster

  4. Department of Molecular Genetics, Ghent University, Technologiepark 927, 9052, Ghent, Belgium

    Nubia Barbosa Eloy, Kristiina Himanen & Gerrit T. S. Beemster

Authors
  1. Cristian Antonio Rojas
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  2. Nubia Barbosa Eloy
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  3. Marcelo de Freitas Lima
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  4. Roberta Lopes Rodrigues
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  7. Gerrit T. S. Beemster
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  8. Adriana Silva Hemerly
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  9. Paulo Cavalcanti Gomes Ferreira
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Correspondence to Paulo Cavalcanti Gomes Ferreira.

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Cristian Antonio Rojas and Nubia Barbosa Eloy contributed equally to this work.

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Rojas, C.A., Eloy, N.B., de Freitas Lima, M. et al. Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size. Plant Mol Biol 71, 307–318 (2009). https://doi.org/10.1007/s11103-009-9525-7

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  • DOI: https://doi.org/10.1007/s11103-009-9525-7

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