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MaSERK1 Gene Expression Associated with Somatic Embryogenic Competence and Disease Resistance Response in Banana (Musa spp.)

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Abstract

A banana somatic embryogenesis receptor-like kinase (SERK) gene, designated as MaSERK1, was isolated from Musa acuminata cv. Mas (AA). It encoded a protein of 628 amino acids with above 82% identities to reported SERKs of coconut, rice, maize, Arabidopsis, carrot, and Medicago truncatula. MaSERK1 was expressed weakly in male flower clusters, but not in male flower-derived nonembryogenic calli, but it was highly expressed in male flower-derived embryogenic calli and embryogenic cell suspensions (ECS). During subculture of ECS, MaSERK1 expression and frequency of somatic embryogenesis were influenced by the duration of subculture, wherein expression decreased within 0 to 6 days of subculture, increased to highest levels at 12 days following subculture, and dropped thereafter. The frequency of somatic embryogenesis of ECS positively correlated with MaSERK1 transcript levels. Moreover, MaSERK1 expression in leaves of Musa paradisiaca L. cv. Dongguan Dajiao (ABB), known to be resistant to Fusarium oxysporum f. sp. cubense race 4 (FOC race 4), was induced by exogenous salicylic acid (SA) or inoculation with FOC race 4. However, MaSERK1 expression levels in leaves of Mucus spp. cv. Pisang awak (ABB), known to be susceptible to FOC race 4, did not change following either treatment. These results suggested that MaSERK1 not only could serve as a molecular marker for banana somatic embryogenesis, but also played a role in disease resistance response in banana.

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Abbreviations

D:

Day

ECS:

Embryogenic cell suspensions

FOC:

Fusarium oxysporum f. sp. cubense

LRR:

Leucine-rich repeat

PCV:

Packed cell volume

R:

Resistance

RACE:

Rapid amplification of cDNA ends

RLK:

Receptor-like kinase

SA:

Salicylic acid

SERK:

Somatic embryogenesis receptor-like kinase

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (30400287), Ministry of Education of China for doctorial study (20050558040), Guangdong Natural Science Foundation (011126, 06023159, 06300470), Programs for Science and Technology Development of Guangdong (2006B20101014), and Programs for Science and Technology Development of Guangzhou (2006Z3-E0281).

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Authors and Affiliations

  1. The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou, 510275, China

    Xia Huang, Xiao-Yan Lu, Jie-Tang Zhao, Jie-Kai Chen, Xue-Mei Dai, Wang Xiao, Ya-Ping Chen, Yun-Feng Chen & Xue-Lin Huang

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  1. Xia Huang
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Correspondence to Xue-Lin Huang.

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Supplementary Figure 1

Multiple full-length amino acid sequence alignment of MaSERK1 with reported somatic embryogenesis receptor-like kinases (SERKs). Putative domains were indicated on the top of the sequences. The eleven subdomains of protein kinase domain were marked with Roman numbers. The asterisks indicated sequence identity. Percentages indicated amino acid identity to MaSERK1. The accession number for SERKs are as follows: CnSERK: AAV58833; OsSERK1: AAU88198; OsBISERK1: AAR26543; ZmSERK1: CAC37638; ZmSERK2: CAC37639; AtSERK1: CAB42254; AtSERK2: AAK68073; AtSERK3: AAK68074; DcSERK: AAB61708; and MtSERK1: AAN64293. Cn = Cocos nucifera; Os = Oryza sativa; Zm = Zea mays; At = Arabidopsis thaliana; Dc = Daucus carota; Mt = Medicago truncatula (PDF 51 kb)

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Huang, X., Lu, XY., Zhao, JT. et al. MaSERK1 Gene Expression Associated with Somatic Embryogenic Competence and Disease Resistance Response in Banana (Musa spp.). Plant Mol Biol Rep 28, 309–316 (2010). https://doi.org/10.1007/s11105-009-0150-z

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