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Cool temperatures interfere with D1 synthesis in tomato by causing ribosomal pausing

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Abstract

Photodamage occurs when leaves are exposed to light in excess of what can be used for photosynthesis and in excess of the capacity of ancillary photoprotective as well as repair mechanisms. An important site of photodamage is the chloroplast encoded D1 protein, a component of the photosystem II (PSII) reaction center. Even under optimal growth irradiance, D1 is photodamaged necessitating rapid turnover to prevent the accumulation of photodamaged PSII reaction centers and consequent inhibition of photosynthesis. However, this on-going process of D1 turnover and replacement was impeded in the chilling-sensitive tomato (Solanum lycopersicum) plants when exposed to high-growth light at cool temperature. The decrease in D1 turnover and replacement was found not to be due to changes in the steady-state level of the psbA message. While the recruitment of ribosomes to psbA transcript, initiation of D1 translation, and the association of polysomes with the thylakoid membrane occurred normally, chilling temperatures caused ribosomal pausing during D1 peptide elongation in tomato. The pause locations were non-randomly located on the D1 transcript. The interference with translation caused by ribosomal pausing allowed photodamaged PSII centers to accumulate leading to the consequent inhibition of photosynthesis.

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Abbreviations

PSII:

Photosystem II

Fv/Fm:

Ratio of variable maximal fluorescence of PSII, represents the dark-adapted maximum efficiency of PSII photochemistry

PPFD:

Photosynthetic photon flux density

RuBP:

Ribulose-1,5-bisphosphate

OEC:

Oxygen-evolving complex

P680:

Reaction center chlorophyll associated with PSII

BBY particles:

Oxygen-evolving PSII preparations

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Authors and Affiliations

  1. Department of Plant Biology, University of Illinois, 1206 W. Gregory Dr., 1407 IGB, Urbana, IL, 61801, USA

    Aleel K. Grennan & Donald R. Ort

  2. Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, IL, 61801, USA

    Donald R. Ort

  3. Department of Crop Science, University of Illinois, Urbana, IL, 61801, USA

    Donald R. Ort

  4. Institute for Genomic Biology, University of Illinois, Urbana, IL, 61801, USA

    Donald R. Ort

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  1. Aleel K. Grennan
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  2. Donald R. Ort
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Correspondence to Aleel K. Grennan.

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Grennan, A.K., Ort, D.R. Cool temperatures interfere with D1 synthesis in tomato by causing ribosomal pausing. Photosynth Res 94, 375–385 (2007). https://doi.org/10.1007/s11120-007-9169-x

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