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Embryo abortion and histological features in the interspecific cross between Phaseolus vulgaris L. and P. coccineus L

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Abstract

In this paper, the causes of early embryo abortion in the reciprocal crosses between Phaseolus vulgaris L. (a cultivar) and Phaseolus coccineus L. (a wild form) were studied. Methacrylate resin sections, 3–5 μm thick, of 3 to 14 day-old seeds were used to examine the embryo developmental stages and the state of seed tissue. It was observed that, embryos aborted at different developmental stages (globular to early cotyledon) depending on the maternal parent. The use of P. coccineus cytoplasm resulted in a higher number of abortion than in reciprocal crosses. Many of them took place between 5 and 6 days after pollination (DAP). Histological analyses permitted to observe that the embryo development was slower in the cross between P. coccineus and P. vulgaris, compared to parental seeds. It would be related to a deficient endosperm development in reciprocal crosses and, in some extent, hypertrophy of the suspensor might be the main cause of early embryo abortion. Then, it would be practical to overcome this incompatibility by rescuing the embryo at the globular stage of development.

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Abbreviations

DAP:

Days after pollination

HEMA:

2-Hydroxyethyl Methacrylate

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Acknowledgements

Thanks to Belgian and Gabonese governments which help us to make the work. We are grateful to Professor Yeung Edward Charles for his advises.

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Authors and Affiliations

  1. Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium

    Pamphile Nguema Ndoutoumou, André Toussaint & Jean Pierre Baudoin

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  1. Pamphile Nguema Ndoutoumou
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  2. André Toussaint
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  3. Jean Pierre Baudoin
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Correspondence to Pamphile Nguema Ndoutoumou.

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Nguema Ndoutoumou, P., Toussaint, A. & Baudoin, J.P. Embryo abortion and histological features in the interspecific cross between Phaseolus vulgaris L. and P. coccineus L. Plant Cell Tiss Organ Cult 88, 329–332 (2007). https://doi.org/10.1007/s11240-006-9198-8

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  • DOI: https://doi.org/10.1007/s11240-006-9198-8

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