Abstract
Jatropha curcas contains high amounts of oil in its seed and has been considered for bio-diesel production. A transformation procedure for J. curcas has been established for the first time via Agrobacterium tumefaciens infection of cotyledon disc explants. The results indicated that the efficiency of transformation using the strain LBA4404 and phosphinothricin for selection was an improvement over that with the strain EHA105 and hygromycin. About 55% of the cotyledon explants produced phosphinothricin-resistant calluses on Murashige and Skoog (MS) medium supplemented with 1.5 mg l−1 benzyladenine (BA), 0.05 mg l−1 3–indolebutyric acid (IBA), 1 mg l−1 phosphinothricin and 500 mg l−1 cefotaxime after 4 weeks. Shoots were regenerated following transfer of the resistant calli to shoot induction medium containing 1.5 mg l−1 BA, 0.05 mg l−1 IBA, 0.5 mg l−1 gibberellic acid (GA3), 1 mg l−1 phosphinothricin and 250 mg l−1 cefotaxime, and about 33% of the resistant calli differentiated into shoots. Finally, the resistant shoots were rooted on 1/2 MS media supplemented with 0.3 mg l−1 IBA at a rate of 78%. The transgenic nature of the transformants was demonstrated by the detection of β-glucuronidase activity in the primary transformants and by PCR and Southern hybridization analysis. 13% of the total inoculated explants produced transgenic plants after approximately 4 months. The procedure described will be useful for both, the introduction of desired genes into J. curcas and the molecular analysis of gene function.
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References
Adebowale KO, Adedire CO (2006) Chemical composition and insecticidal properties of the underutilized Jatropha curcas seed oil. Afr J Biotech 10:901–906
Banerji R, Chowdhury AR, misra G, Sudarsanam G, Verma SC, Srivastava GS (1985) Jatropha curcas seed oils for energy. Biomass 8:277–282
Brasileiro ACM, Aragao FJL (2001) Marker genes for in vitro selection of transgenic plants. J Plant Biotechnology 3(3):113–121
Chen Y-X, Mao Z-Q, Wu Z-B, Zhu H-P, Tang Z-Y (2006) Comprehensive exploitation and utilization of Jatropha oil plants. China Oils Fats 31(3):63–65
Dai S, Zheng P, Marmey P, Zhang S, Tian W, Chen S, Beachy RN, Fauquet C (2001) Comparative analysis of transgenic rice plants obtained by Agrobacterium mediated transformation and particle bombardment. Mol Breed 7:25–33
De Block M, Botterman J, Vandewiele M, Dodkx J, Thoen C, Gossele V, Rao Movva N, Thompson C, Van Montagu M, Leemans J (1987) Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J 6:2513–2518
De Winnaar W (1988) Clonal propagation of papaya in vitro. Plant Cell Tiss Org Cult 12:305–310
Forson FK (2004) Performance of Jatropha oil blends in a diesel engine. Renewable Energy 29:1135–1145
Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the “Gene-Jockeying” tool. Micro Mol Bio Rev 67:16–37
Gubitz GM, Mittelbach M, Trabi M (1999) Exploitation of the tropical seed plant Jatropha curcas L. Bioresource Tech 67:73–82
Gupta RC (1985) Pharmacognostic studies on Dravanti: Jatropha curcas. Plant Sci 94:65–82
Hébert-Soulé D, Kikkert JR, Reisch BI (1994) Phosphinothricin stimulates somatic embryogenesis in grape (Vitis sp. L.). Plant cell Rep 14:380–384
Höfgen R, Willmitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16:9877
Hussain TM, Chandrasekhar T, Arifullah M, Gopal GR (2004) Effect of benzyladenine and thidiazuron on in vitro shoot formation from cotyledonary nodes of Tramarindus indica Linn. Propagation Orn. Plants 4:47–52
Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
Jefferson RA, Kavangh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
Karim MZ, Amin MN, Azad MAK, Begum F, Rahman MM, Islam MM, Alam R (2003) Effects of different plant growth regulator on in vitro shoot multiplication of Chrysanthemum morifolium. OnLine J Bio Sci 3:553–560
Keith O (2000) A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass & Bioenergy 19:1–15
Li K, Yang W-Y, Li L, Zhang C-H, Cui Y-Z, Sun Y-Y (2007) Distribution and development strategy for Jatropha curcas L. For Stud China 9(2):120–126
Li MR, Li HQ, Wu GJ (2006) Study on factors influencing Agrobacterium-mediated transformation of Jatropha curcas. J Mol Cell Bio 39:83–87
Li WL, Yang H (2001) Research on a recycling energy plant J. curcas seed oil chemical component analysis. Yunnan Univ. J (Nat Sci Ed) 22(5):324–330
Lin J, tang L, Chen F (2002) Tissue culture and plantlet regeneration of Jatropha curcas. Plant Physiol Commun 38:252
Lin J, Zhou XW, Tang KX, Chen F 2004) A survey of the studies on the resources of Jatropha curcas. J Trop Subtrop Bot 12:285–290
Lu WD, Wei Q, Tang L, Yan F, Chen F (2003) Induction of callus from Jatropha curcas and rapid propagation. Chin J Appl Environ Biol 9:127–130
Mampane KJ, Joubert PH, Hay IT (2006) Jatropha curcas: use as a traditional Tswana medicine and its role as a cause of acute poisoning. Phytotherapy Res 1:50–51
Martin G, Mayeux A (1985) Curcas oil (Jatropha curcas L.): a possible fuel. Agr Troop 9:73–75
Mathur A, Mathur AK, Gangwar A (2001) In vitro plantlet regeneration in Panax sikkimensis. Planta Med 67:181–183
Mohamed ME, Hicks RGT, Blakesley D (1996) Shoot regeneration from mature endosperm of Passiflora foetida. Plant Cell Tiss Org Cult 44:161–164
Muhammad AL, Ye G-N, Weeden NF, Reisch BI (1994) A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Ampelopsis. Plant Mol Bio Rep 12:6–13
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Openshaw K (2000) A review of Jatropha curcas: an oil plant unfulfilled promise. Biomass Bioenergy 19:1–15
Stafford HA (2000) Crown gall disease and Agrobacterium tumefaciens: a study of the history, present knowledge, missing information, and impact on molecular genetics. Bot Rev 66:99–118
Staubmann R, Ncube I, Gubitz GM, Steiner W, Read JS (1999) Esterase and lipase activity in Jatropha curcas L. J Biotech 75:117–126
Sujatha M, Mukta N (1996) Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tiss Org Cult 44:135–141
Thorpe TA (1983) Morphologenesis and regeneration in tissue culture. Beltsville Sym Agr Res 7:285–303
Travella S, Ross SM, Harden J, Everett C, Snape JW, Harwood WA (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Rep 23:780–789
Valvekens D, Montagu MV, Lijsebettens MV (1988) Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci USA 85:5536–5540
Wei Q, Lu W-D, Liao Y, Pan S-L, Xu Y, Tang L, Chen F (2004) Plant regeneration from epicotyl explant of Jatropha curcas. J Plant Physiol Mol Bio 30:475–478
Zhou H, Lu H, Liang B (2006) Solubility of multicomponent systems in the biodiesel production by transesterification of Jatropha curcas L. oil with methanol. J Chem Eng Data 51:1130–1135
Acknowledgements
This research was supported by the Key Innovation Programs of the Chinese Academy of Sciences (KSCX2-YW-G-027), Science and Technology of Guangdong province (2005B20801009; 2006B20201009) and The CAS “100 Talents” program. The authors are grateful to Dr. Christian Walter and the anonymous referees for their useful comments and valuable suggestions to improve the paper.
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Li, M., Li, H., Jiang, H. et al. Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas . Plant Cell Tiss Organ Cult 92, 173–181 (2008). https://doi.org/10.1007/s11240-007-9320-6
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DOI: https://doi.org/10.1007/s11240-007-9320-6