Abstract
The isolation and expression analysis of four partial gene sequences from rose (Rosa hybrida cv. Linda) belonging to the receptor-like kinase gene superfamily are reported. These genes have been designated RhSERK1 to RhSERK4 (Accession No. EF631967 to EF631970) as they exhibit high sequence identities with genes from the somatic embryogenesis receptor-like kinase (SERK) family in other plant species. The RhSERK genes are differentially expressed in non-embryogenic callus, embryogenic callus, mature somatic embryos and a range of tissues from intact plants, indicating a broad role in plant growth and development. However, the expressions of RhSERK3 and RhSERK4 were approximately fivefold higher in embryogenic callus than in non-embryogenic callus, and they are even higher when compared to tissues from intact plants. In addition, RhSERK4 expression was approximately eightfold higher in somatic embryos than in embryogenic callus. These results suggest that the expression pattern of RhSERK3 and RhSERK4 may be used as a marker of somatic embryogenesis.
Similar content being viewed by others
Abbreviations
- NCBI:
-
National center for biotechnology information
- RLK:
-
Receptor-like kinase
- SERK:
-
Somatic embryogenesis receptor-like kinase
References
Albrecht C, Russinova E, Hecht V, Baaijens E, de Vries SC (2005) The Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES 1 and 2 control male sporogenesis. Plant Cell 17:3337–3349
Albrecht C, Russinova E, Kemmerling B, Kwaaitaal M, de Vries SC (2008) Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE proteins serve brassinosteroid-dependent and -independent signaling pathways. Plant Physiol 148:611–619
Baudino S, Hansen S, Brettschneider R, Hecht VFG, Dresselhaus T, Lorz H, Dumas C, Rogowsky PM (2001) Molecular characterization of two novel maize LRR receptor-like kinases, which belong to the SERK gene family. Planta 213:1–10
Burrell AM, Lineberger RD, Rathore KS, Byrne DH (2006) Genetic variation in somatic embryogenesis of rose. HortScience 41:1165–1168
Chugh A, Khurana P (2002) Gene expression during somatic embryogenesis—recent advances. Curr Sci 83(6):715–730
Cock JM, Vanoosthuyse V, Gaude T (2002) Receptor kinase signalling in plants and animals: distinct molecular systems with mechanistic similarities. Curr Opin Cell Biol 14:230–236
Estabrooks T, Browne R, Dong Z (2007) 2,4,5-Trichlorophenoxyacetic acid promotes somatic embryogenesis in the rose cultivar ‘Livin’ Easy’ (Rosa sp.). Plant Cell Rep 26:153–160
Feher A, Pasternak TP, Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue Organ Cult 74:201–228
He K, Gou X, Yuan T, Lin H, Asami T, Yoshida S, Russell SD, Li J (2007) BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr Biol 17:1109–1115
Hecht V, Vielle-Calzada JP, Hartog MV, Schmidt DL, Boutilier K, Grossnicklaus U, de Vries SC (2001) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol 127:803–816
Heese A, Hann DR, Gimenez-Ibanez S, Jones AME, He K, Li J, Schroeder JI, Peck SC, Rathjen JP (2007) The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc Natl Acad Sci USA 104:12217–12222
Ito Y, Takaya K, Kurata N (2005) Expression of SERK family receptor-like protein kinase genes in rice. Biochim Biophys Acta 1730:253–258
Jimenez VM (2005) Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul 47:91–110
Karlova R, Boeren S, Russinova E, Aker J, Vervoort J, de Vries S (2006) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 protein complex includes BRASSINOSTEROID-INSENSITIVE1. Plant Cell 18:626–638
Li J, Wen J, Lease KA, Doke JT, Tax FE, Walker JC (2002) BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell 110:213–222
Müller R, Lind-Iversen S, Stummann BM, Serek M (2000a) Expression of genes for ethylene biosynthetic enzymes and an ethylene receptor in senescing flowers of miniature roses. J Hortic Sci Biotech 75:12–18
Müller R, Stummann BM, Serek M (2000b) Characterization of an ethylene receptor family with differential expression in rose (Rosa hybrida L.) flowers. Plant Cell Rep 19:1232–1239
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco callus. Physiol Plant 15:473–497
Nolan KE, Irwanto RR, Rose RJ (2003) Auxin up-regulates MtSERK1 expression in both Medicago trancatula root-forming and embryogenic cultures. Plant Physiol 133:218–230
Pérez-Núnez MT, Souza R, Sáenz L, Chan JL, Zúniga-Aguilar JJ, Oropeza C (2009) Detection of a SERK-like gene in coconut and analysis of its expression during the formation of embryogenic callus and somatic embryos. Plant Cell Rep 28:11–19
Raemakers CJJM, Jacobsen E, Visser RGF (1995) Secondary somatic embryogenesis and application in plant breeding. Euphytica 81:93–107
Santos MO, Romano E, Yotoko KSC, Tinoco MLP, Dias BBA, Aragão FJL (2005) Characterization of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis. Plant Sci 168:723–729
Santos MA, Romano E, Vieira LS, Baldoni AB, Aragão FJL (2009) Suppression of SERK gene expression affects fungus tolerance and somatic embryogenesis in transgenic lettuce. Plant Biol 11:83–89
Schmidt EDL, Guzzo F, Toonen MAJ, de Vries SC (1997) A leucine- rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124:2049–2062
Sharma SK, Millam S, Hein I, Bryan GL (2008) Cloning and molecular characterization of a potato SERK gene transcriptionally induced during initiation of somatic embryogenesis. Planta 228:319–330
Shimada T, Hirabayashi T, Endo T, Fujii H, Kita M, Omura M (2005) Isolation and characterization of the somatic embryogenesis receptor-like kinase gene homologue (CitSERK1) from Citrus unshiu Marc. Sci Hortic 103:233–238
Somleva MN, Schmidt EDL, de Vries SC (2000) Embryogenic cells in Dactylis glomerata L. (Poaceae) explants identified by cell tracking and by SERK expression. Plant Cell Rep 19:718–726
Staba JE (1969) Plant tissue culture as a technique for the phytochemist. Recent Adv Phytochem 2:75–106
Tatusova TA, Madden TL (1999) Blast 2 sequences—a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174:247–250
Thomas C, Meyer D, Himber C, Steinmetz A (2004) Spatial expression of a sunflower SERK gene during induction of somatic embryogenesis and shoot organogenesis. Plant Physiol Biochem 42:35–42
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Ye X, Kang B, Osburn LD, Cheng ZM (2009a) The COBRA gene family in Populus and gene expression in vegetative organs and in response to hormones and environmental stresses. Plant Growth Regul 58:211–223
Ye X, Kang B, Osburn LD, Li Y, Cheng ZM (2009b) Identification of the flavin-dependent monooxygenase-encoding YUCCA gene family in Populus trichocarpa and their expression in vegetative tissues and in response to hormone and environmental stresses. Plant Cell Tiss Organ Cult 97:271–283
Zakizadeh H, Debener T, Sriskandarajah S, Frello S, Serek M (2008) Regeneration of miniature potted rose (Rosa hybrida L.) via somatic embryogenesis. Eur J Hortic Sci 73:111–117
Acknowledgements
We are grateful to Prof. Brian Grout for linguistic correction of the manuscript. This research was funded by the Ministry of Science, Research and Technology of Iran.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zakizadeh, H., Stummann, B.M., Lütken, H. et al. Isolation and characterization of four somatic embryogenesis receptor-like kinase (RhSERK) genes from miniature potted rose (Rosa hybrida cv. Linda). Plant Cell Tiss Organ Cult 101, 331–338 (2010). https://doi.org/10.1007/s11240-010-9693-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-010-9693-9