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Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine

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Abstract

The present study was carried out to explore the seroprevalence of brucellosis in yaks of North-Eastern hilly yak tracts of Arunachal Pradesh, India. Of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin ELISA (AB-ELISA), Rose-Bengal plate test (RBPT) and standard tube-agglutination test (STAT), respectively. The relative sensitivity and specificity for STAT were 79.77 and 100%, respectively and the same for RBPT were 88.76 and 100%, respectively in comparison to AB-ELISA. The alarming prevalence as recorded was highest among the yak cows (31.42%) followed by heifers (23.85%) and bulls (8.88%). The immune response in yaks following standard dose of calfhood vaccination with Brucella abortus strain 19 vaccine showed that protective antibody level persisted up to 210 days. This is the first report from India on prevalence of brucellosis and immunization with B abortus strain 19 vaccine in yaks. The present investigation would be a valuable guideline for future control measure and eradication programme of brucellosis in yaks.

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Abbreviations

AB-ELISA:

avidin-biotin enzyme-linked immunosorbent assay

FPSR:

false positive serological cross-reactions

HRPO:

horseradish peroxidase

IVRI:

Indian Veterinary Research Institute

OD:

optical density

OPD:

orthophenylene diamine dihydrochloride

PBS:

phosphate buffer saline

PD_ADMAS:

Project Directorate of Animal Disease Monitoring and Surveillance

PP:

percent positivity

RBPT:

rose bengal plate test

STAT:

standard tube-agglutination test

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Authors and Affiliations

  1. National Research Centre on Yak, Dirang, Arunachal Pradesh, 790 101, India

    Samiran Bandyopadhyay, Debasis Sasmal, Monoj Kumar Ghosh, Mihir Sarkar & Mohan Bhattacharya

  2. Central Agricultural University, Selesih, Aizwal, India

    Tapan Kumar Dutta

  3. Department of Parasitology, West Bengal University of Animal and Fishery Sciences, 37, Belgachia Road, Kolkata, 700 037, West Bengal, India

    Nihar Kanta Sasmal

Authors
  1. Samiran Bandyopadhyay
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  2. Debasis Sasmal
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  3. Tapan Kumar Dutta
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  4. Monoj Kumar Ghosh
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  5. Mihir Sarkar
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  6. Nihar Kanta Sasmal
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  7. Mohan Bhattacharya
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Correspondence to Nihar Kanta Sasmal.

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Bandyopadhyay, S., Sasmal, D., Dutta, T.K. et al. Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine. Trop Anim Health Prod 41, 587–592 (2009). https://doi.org/10.1007/s11250-008-9228-0

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  • DOI: https://doi.org/10.1007/s11250-008-9228-0

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