Abstract
Purpose
Nigeria is the largest cattle-rearing nation in Africa with most animals kept under traditional husbandry practices. While bovine brucellosis does not receive much attention, a relatively high seroprevalence is found in samples submitted for laboratory testing. The aim of the study was to provide serological evidence of brucellosis in cattle from some of the main cattle-rearing states of the country and to validate a simple and rapid field test for the serodiagnosis of bovine brucellosis.
Method
Serum samples collected in various states of Nigeria from cattle because of suspicion of brucellosis were investigated in the Rose Bengal plate test, and results were compared with a newly developed rapid field test for the detection of Brucella-specific antibodies.
Results
Serological evidence for the presence of brucellosis in cattle was obtained for all states included in the study and a high herd prevalence was observed. The seroprevalence was also high among trade and slaughter animals. Results of a rapid field test for the serodiagnosis of bovine brucellosis correlated well with the Rose Bengal plate test (agreement, 95.7%; kappa value, 0.80).
Conclusions
The results indicate that bovine brucellosis is an important veterinarian problem in Nigeria. The easy-to-use and robust field test is most promising for field-based surveillance as it provides an immediate result allowing the prompt instigation of control measures.
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References
Abdoel, T., Dias, I.T., Cardoso, R. and Smits, H.L., 2008. Simple and rapid field tests for brucellosis in livestock, Veterinary Microbiology, 130, 312–319.
Alton, G.G., Jones, L.M., Angus, R.D. and Verger, J.M., 1988.Techniques for the brucellosis laboratory, Institute National de le Recherche Agronomique, Paris, France. pp 63-129.
Bayemi, P.H., Webb, E.C., Nsongka, M.V., Unger, H. and Njakoi, H., 2009. Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon, Tropical Animal Health and Production, 41, 141–144.
Bronsvoort, B.M., Koterwas, B., Land, F., Handel, I.G., Tucker, J., Morgan, K.L., Tanya, V.N., Abdoel, T.H. and Smits, H.L., 2009. Comparison of a flow assay for brucellosis antibodies with the reference cELISA test in West African Bos indicus, PLoS One, 4, e5221.
Cadmus, S.I., Adesokan, H.K., Adedokun, B.O. and Stack, J.A., 2010. Seroprevalence of bovine brucellosis in trade cattle slaughtered in Ibadan, Nigeria, from 2004–2006, Journal South African Veterinary Association, 81, 50–53.
Chimana, H.M., Muma, J.B., Samui, K.L., Hangombe, B.M., Munyeme, M., Matope, G., Phiri, A.M., Godfroid, J., Skjerve, E. and Tryland, M. 2010. A comparative study of the seroprevalence of brucellosis in commercial and small-scale mixed dairy-beef cattle enterprises of Lusaka province and Chibombo district, Zambia, Tropical Animal Health and Production, 42, 1541–1545.
Godfroid, J., Scholz, H.C., Barbier, T., Nicolas, C., Wattiau, P., Fretin, D., Whatmore, A.M., Cloeckaert, A., Blasco, J.M., Moriyon, I., Saegerman, C., Muma, J.B., Al Dahouk, S., Neubauer, H. and Letesson, J.J. 2011. Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century, Preventive Veterinary Medicine doi:10.1016/j.prevetmed.2011.04.007
Matope, G., Bhebhe, E., Muma, J.B., Oloya, J., Madekurozwa, RL, Lund, A and Skjerve E. 2011. Seroprevalence of brucellosis and its associated risk factors in cattle from smallholder dairy farms in Zimbabwe, Tropical Animal Health and Production, 43, 975–982
Megersa, B., Biffa, D., Niguse, F., Rufael, T., Asmare, K. and Skjerve, E. 2011. Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication, Acta Veterinaria Scandinavia, 53, 24.
Ocholi, R.A., Kwaga, J.K., Ajogi, I. and Bale, J.O. 2004. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria, Veterinary Microbiology, 103, 47–53.
Pappas, G., Papadimitriou, P., Akritidis, N., Christou, L. and Tsianos, EV., 2006. The new global map of human brucellosis, Lancet Infectious Disease, 6, 91–99.
Schelling, E., Diguimbaye, C., Daoud, S., Nicolet, J., Boerlin, P., Tanner, M. and Zinsstag, J., 2003. Brucellosis and Q-fever seroprevalences of nomadic pastoralists and their livestock in Chad, Preventive Veterinary Medicine, 61, 279–293.
Scolamacchia F., Handel I.G., Fèvre E.M., Morgan K.L., Tanya V.N. and Bronsvoort B.M., 2010. Serological patterns of brucellosis, leptospirosis and Q fever in Bos indicus cattle in Cameroon, PLoS One, 5, e8623.
Tesfaye, G., Tsegaye, W., Chanie, M. and Abinet, F. 2011. Seroprevalence and associated risk factors of bovine brucellosis in Addis Ababa dairy farms, Tropical Animal Health and Production, 43, 1001–1005.
Zinsstag J., Roth F., Orkhon D., Chimed-Ochir G., Nansalmaa M., Kolar J. and Vounatsou P. (2005). A model of animal-human brucellosis transmission in Mongolia, Preventive Veterinary Medicine, 69, 77–95.
Acknowledgment
The authors like to thank Dr. L.H. Lombin, the executive director, and Dr. D. Shamaki, director of Research of the National Veterinary Research Institute, for their full support for this study.
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Bertu, W.J., Gusi, A.M., Hassan, M. et al. Serological evidence for brucellosis in Bos indicus in Nigeria. Trop Anim Health Prod 44, 253–258 (2012). https://doi.org/10.1007/s11250-011-0011-2
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DOI: https://doi.org/10.1007/s11250-011-0011-2