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Isolation and phylogenetic analysis of avian-origin European H1N1 swine influenza viruses in Jiangsu, China

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Abstract

Isolates of the A(H1N1)pdm2009 virus were first identified in asymptomatic swine in Jiangsu province, China in January 2010, indicating that the virus has retro-infected swine after circulating through humans in mainland China. The purpose of this study was to determine whether the avian-origin European H1N1 swine influenza virus (SIV) and the A(H1N1)pdm2009 virus are cocirculating in swine in Jiangsu province of China. From May 2010 to May 2011, 1,030 nasal swab samples were collected from healthy swine in Jiangsu province of China and were tested for influenza A H1N1 using reverse transcription-PCR. Fragments of the complete genomes of viruses from the samples that were positive for influenza A H1N1 were sequenced and analysed. A total of 32 avian-origin European H1N1 SIVs were isolated, and no A(H1N1)pdm2009 viruses were identified; full-length genomes of 18 strains were sequenced. The eight gene segments of some of the isolated H1N1 viruses have 99.1–99.8% sequence identity with the human A/Jiangsu/ALS1/2011(H1N1) isolates in the same region. Our study indicates that the avian-origin European H1N1 SIVs remain endemic in swine and have retro-infected humans after circulating through swine, which may present a risk factor for public health.

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Acknowledgments

This study was supported by the Major State Basic Research Development Program (973 Program) (No. 2011CB505003), the Important National Science & Technology Specific Projects (Nos. 2008ZX10004-013 and 2009ZX10004-214), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, People’s Republic of China

    Guo Zhao, Jinjin Pan, Xiaobing Gu, Xinlun Lu, Qunhui Li, Jie Zhu, Chaoyang Chen, Zhiqiang Duan, Quangang Xu, Xiaobo Wang, Shunlin Hu, Wenbo Liu, Daxin Peng, Xiaowen Liu, Xiaoquan Wang & Xiufan Liu

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  1. Guo Zhao
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  2. Jinjin Pan
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  3. Xiaobing Gu
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  4. Xinlun Lu
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  6. Jie Zhu
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  14. Xiaowen Liu
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  15. Xiaoquan Wang
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  16. Xiufan Liu
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Correspondence to Xiufan Liu.

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Zhao, G., Pan, J., Gu, X. et al. Isolation and phylogenetic analysis of avian-origin European H1N1 swine influenza viruses in Jiangsu, China. Virus Genes 44, 295–300 (2012). https://doi.org/10.1007/s11262-011-0704-7

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  • DOI: https://doi.org/10.1007/s11262-011-0704-7

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