Summary
Production of poly(3-hydroxybutyric acid) [P(3HB)] by Rhodopseudomonas palustris SP5212 isolated in this laboratory has been optimized under phototrophic microaerophilic conditions. Cells grown in malate medium accumulated 7.7% (w/w) P(3HB) of cellular dry weight at the early stationary phase of growth. The accumulated P(3HB) however, attained 15% (w/w) of cellular dry weight when acetate (1.0%, w/v) was used as the sole carbon source under nitrogen-limiting conditions. Synthesis and accumulation of polymer was favoured by sulphate-free conditions and at a phosphate concentration sub-optimal for growth. The polymer content of cells was increased drastically (34% of cellular dry weight) when the acetate containing medium was supplemented with n-alkanoic acids. Compositional analysis by H1 NMR revealed that these accumulated polymers were composed of 3-hydroxybutyric acid and 3-hydroxyvaleric acid (3HV). The contents of 3HV in these copolymers ranged from 14 to 38 mol%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A.J. Anderson E.A. Dawes (1990) ArticleTitleOccurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates Microbiological Reviews 54 450–472 Occurrence Handle2087222
E.J. Bormann M. Leibner B. Beer (1998) ArticleTitleGrowth associated production of polyhydroxybutyric acid by Azotobacter beijerinckii from organic nitrogen substrate Applied Microbiology and Biotechnology 49 84–88
H. Brandl E.J. Knee SuffixJr. R.C. Fuller R.A. Gross R.W. Lenz (1989) ArticleTitleAbility of the phototrophic bacterium Rhodospirillum rubrum to produce various poly(β-hydroxyalkanoates): potential sources for biodegradable polyesters International Journal of Biological Macromolecules 11 49–55 Occurrence Handle2518731
H. Brandl R.A. Gross R.W. Lenz R. Llyod R.C. Fuller (1991) ArticleTitleThe accumulation of poly(3-hydroxyalkanoates) in Rhodobacter sphaeroides Archives of Microbiology 155 337–340
D. Byrom (1987) ArticleTitlePolymer synthesis by microorganisms: technology and economics Trends in Biotechnology 5 246–250
E.A. Dawes P.J. Senior (1973) ArticleTitleThe role of energy reserve polymers in microorganisms Advances in Microbial Physiology 10 135–266 Occurrence Handle4594739
K. Hashimoto H. Tsuboi S. Iwasaki Y. Shirai (1993) ArticleTitleEffect of pH on the production of PHB by photosynthetic bacteria Rhodospirillum rubrum Journal of Chemical Engineering of Japan 26 56–58
E. Khatipov J. Miyake Y. Asada (1998) ArticleTitleAccumulation of poly-β- hydroxybutyrate by Rhodopseudomonas sphaeroides on various carbon and nitrogen substrates FEMs Microbiology Letters 162 39–45
J.H. Law R.A. Sleepecky (1961) ArticleTitleAssay of poly-β-hydroxybutyric acid Journal of Bacteriology 82 33–36 Occurrence Handle13759651
M. Libergesell E. Hustede A. Timm A. Steinbuchel R.C. Fuller R.W. Lenz H.G. Schlegel (1991) ArticleTitleFormation of poly(3-hydroxyalkanoic) acids by phototrophic and lithotrophic bacteria Archives of Microbiology 155 415–421
A. Manna A.K. Paul (2003) ArticleTitleGrowth associated production and characterization of poly(3-hydroxybutyric acid) of Azotobacter beijerinckii DAR 102 Indian Journal of Experimental Biology 41 129–134 Occurrence Handle15255604
W.J. Page O. Knosp (1989) ArticleTitleHyperproduction of poly-β-hydroxy butyrate during exponential growth of Azotobacter vinelandii Applied and Environmental Microbiology 55 1334–1339
S. Pal A. Manna A.K. Paul (1998) ArticleTitleNutritional and cultural conditions for production of poly-3-hydroxybutyric acid by Azotobacter chroococcum Folia Microbiologica 43 177–181
H.G. Schlegel (1962) ArticleTitleDie specicherstoffe van Chromatium okenii. Archiv für Mikrobiologie 42 110–116 Occurrence Handle14498518
P.J. Senior G.A. Beech A.F. Ritchie E.A. Dawes (1972) ArticleTitleThe role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii Biochemical Journal 128 1193–1201 Occurrence Handle4643700
H.W. Ulmer R.A. Gross M. Posada P. Weisbach R.C. Fuller R.W. Lenz (1994) ArticleTitleBacterial production of poly(β-hydroxyalkanoates) containing unsaturated repeating units by Rhodospirillum rubrum Macromolecules 27 1675–1679
M. Vincenzini C. Sili R. Phillips Particlede A. Ena R. Materassi (1990) ArticleTitleOccurrence of poly-β-hydroxybutyrate in Spirullina species Journal of Bacteriology 172 2791–2797 Occurrence Handle2332408
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mukhopadhyay, M., Patra, A. & Paul, A.K. Production of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Rhodopseudomonas palustris SP5212. World J Microbiol Biotechnol 21, 765–769 (2005). https://doi.org/10.1007/s11274-004-5565-y
Issue Date:
DOI: https://doi.org/10.1007/s11274-004-5565-y