Skip to main content
SpringerLink
Log in

Enhancement of stability of biosurfactant produced by Candida lipolytica using industrial residue as substrate

  • Technical Communicatiosn
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

This work describes experimental results carried out on the fermentation of Candida lipolytica, which produced a new biosurfactant when grown on a vegetable oil refinery residue as substrate. The cell-free culture broth containing the biosurfactant formed stable emulsions with hydrophobic natural compounds. Emulsification properties of the biosurfactant were not affected by salinity; however, treatment at a higher temperature decreased the emulsification activity, indicating applications in oil recovery. The isolated biosurfactant corresponds to a yield of 4.5 g/l, and the surface tension of water was reduced from 71 to 32 mN/m. Preliminary chemical characterizations showed that the biosurfactant consisted of protein (50%), lipid (20%), and carbohydrate (8%).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

References

  • Abu-Ruwaida AS, Banat IM, Haditirto S et al (1991) Isolation of biosurfactant-producing bacteria—product characterization, and evaluation. Acta Biotechnol 2:315–324

    Article  Google Scholar 

  • Amaral PFF, da Silva JM, Lehocky M et al (2006) Production and characterization of bioemulsifier from Yarrowia lipolytica. Process Biochem 41:1894–1898

    Article  CAS  Google Scholar 

  • Arbige MV, Pitcher WM (1989) Industrial enzymology: a look towards the future. Trends Biotechnol 7:330–335

    Article  CAS  Google Scholar 

  • Batista SB, Mounteer AH, Amorim FR, Tótola MR (2006) Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresour Technol 97:868–875

    Article  CAS  Google Scholar 

  • Bednarski W, Adamczak M, Tomasik J et al (2004) Application of oil refinery waste in the biosynthesis of glycolipids by yeast. Bioresour Technol 95:15–18

    Article  CAS  Google Scholar 

  • Cirigliano MC, Carman GM (1984) Isolation of a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 48:747–750

    CAS  Google Scholar 

  • Cirigliano MC, Carman GM (1985) Purification and characterization of liposan, a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 50:846–850

    CAS  Google Scholar 

  • Cooper DG, Goldenberg BG (1987) Surface active agents from two Bacillus species. Appl Environ Microbiol 53:224–229

    CAS  Google Scholar 

  • Cooper DG, Zajic JE (1980) Surface active compounds from microorganisms. Adv Appl Microbiol 26:229–253

    Article  CAS  Google Scholar 

  • Costa SGVAO, Nitschke M, Haddad R et al (2005) Production Pseudomonas aeruginosa LBI rhamnolipids following growth on Brazilian native oils. Process Biochem 21:1593–1600

    Google Scholar 

  • Desai JD, Desai AJ (1993) Production of biosurfactnt. In: Kosaric N (ed) Biosurfactant: production, properties, applications, vol 48. Surfactant Science Series. Marcel Dekker Inc., New York, pp65–97

  • Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64

    CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK (1956) Colorimetric method for determination of sugar and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Foght JM, Gutnick DL, Westlake DWS (1989) Effect of emulsion on biodegradation of crude oil by pure and mixed cultures. Appl Environ Microbiol 55:36–42

    CAS  Google Scholar 

  • Francy DS, Thomas JM, Raymand RL et al (1991) Emulsification of hydrocarbons by surface bacteria. J Ind Microbiol 8:237–246

    Article  CAS  Google Scholar 

  • Ghurye GL, Vipulanandan C, Willson RC (1994) A practical approach to biossurfactant production using nonaseptic fermentation of mixed cultures. Biotechnol Bioeng 44:661–666

    Article  CAS  Google Scholar 

  • Haba E, Espuny MJ, Busquets M et al (2000) Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2 NCIB 40044 from waste frying oils. J Appl Microbiol 88:379–387

    Article  CAS  Google Scholar 

  • Makkar RS, Cameotra SS (2002) An update on the use of unconventional substrates for biosurfactant production and their new applications. Appl Microbiol Biotechnol 58:428–434

    Article  CAS  Google Scholar 

  • Manocha MS, San-Blas G, Centeno S (1980) Lipid composition of Paracoccidioides brasilienses: possible correlation with virulence of different strains. J Gen Microbiol 117:147–154

    CAS  Google Scholar 

  • Miller RM, Zhang Y (1992) Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl Environ Microbiol 58:3276–3282

    Google Scholar 

  • Miranda OA, Salgueiro AA, Pimentel MCB et al (1999) Lipase production by a Brazilian strain of Penicillium citrinum using an industrial residue. Bioresour Technol 69:145–147

    Article  CAS  Google Scholar 

  • Nitschke M, Pastore GM (2002) Biosurfactantes: propriedades e aplicações. Quim Nova 25:772–776

    CAS  Google Scholar 

  • Pekin G, Vardar-Sukan F (2006) Production of sophorolipids using the yeast Candida bombicola ATTC22214 for the applications in the food industry. J Eng Nat Sci 2:109–116

    Google Scholar 

  • Ron EZ, Rosenberg E (2001) Natural roles of biosurfactants. Environ Microbiol 3:229–236

    Article  CAS  Google Scholar 

  • Rufino RD, Luna JM, Amorim AS et al (2003) Biosurfactant production by Candida lipolytica using an industrial residue as substrate. In: Abstracts of the 2nd European Bioremediation Conference, Chania, Crete, Greece, 30 June –4 July, 2003

  • Sarubbo LA, Porto ALF, Campos-Takaki GM (1999) The use of babassu oil as substrate to produce bioemulsifiers by Candida lipolytica. Can J Microbiol 45:423–426

    Article  CAS  Google Scholar 

  • Sarubbo LA, Marçal MC, Neves MLC et al (2001) Bioemulsifier production in batch culture using glucose as carbon source by Candida lipolytica. Appl Biochem Biotechnol 95:59–67

    Article  CAS  Google Scholar 

  • Zhou QH, Kosaric N (1995) Utilization of canola oil and lactose to produce biosurfactant with Candida bombicola. J Am Oil Chem Soc 72:67–71

    CAS  Google Scholar 

Download references

Acknowledgments

This work was carried out with the financial support of FINEP/CTPETRO, FACEPE, and CNPq.

Author information

Authors and Affiliations

  1. Fungal Biology, Federal University of Pernambuco, Recife-Pernambuco, Brazil

    R. D. Rufino

  2. Chemistry Department, Catholic University of Pernambuco, Rua do Príncipe, n. 526, Boa Vista, CEP 50.050-900, Recife-PE, Brazil

    L. A. Sarubbo & G. M. Campos-Takaki

  3. Nucleus of Research in Environmental Sciences (NPCIAMB), Catholic University of Pernambuco, Rua Nunes Machado, no. 42, Bl J, Boa Vista, 50.050-590 , Recife-PE, Brazil

    R. D. Rufino & L. A. Sarubbo

Authors
  1. R. D. Rufino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. A. Sarubbo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. M. Campos-Takaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. M. Campos-Takaki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rufino, R.D., Sarubbo, L.A. & Campos-Takaki, G.M. Enhancement of stability of biosurfactant produced by Candida lipolytica using industrial residue as substrate. World J Microbiol Biotechnol 23, 729–734 (2007). https://doi.org/10.1007/s11274-006-9278-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-006-9278-2

Keywords

Search

Navigation