Abstract
The levels of chromosome instability and heat absorption of chromatin have been studied in cultured lymphocytes derived from blood of 80–93- and 18–30-year-old individuals, under the effect of heavy metal Cu(II) and Cd(II) salts. The analysis of the results obtained indicates that 50 μM Cu(II) induced a significantly higher level of cells with chromosome aberrations in old donors (13.8 ± 1.5% vs control, 3.8 ± 1.7%), whereas treatment with 100 μM Cd(II) did not induce any changes in the background index. Analysis of the lymphocyte melting curves showed that Cu(II) ions caused more effective condensation of heterochromatin in old healthy individuals compared with young donors, which was expressed by the increase of the T m of elderly chromatin by ~3°C compared with the norm. Treatment of lymphocyte chromatin of old individuals with 100 μM Cd(II) caused decondensation (deheterochromatinization) of both the facultative and constitutive domains of heterochromatin. The deheterochromatinization T m was decreased by ~3–3.5°C compared with the T m observed for young individuals. Thus, the chromatin of cultured lymphocytes from the old-aged individuals underwent modification under the influence of copper and cadmium salts. Cu(II) caused additional heterochromatinization of heterochromatin, and Cd(II) caused deheterochromatinization of facultative and constitutive heterochromatin. Our data may be important as new information on the remodeling of constitutive and facultative heterochromatin induced by heavy metals in aging, aging pathology, and pathology linked with metal ions.
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References
Berger NA, Eichhorn GL (1971) Interaction of metal ions polynucleotides and related compounds. 18. The multiplicity of reactions of copper (II) with inozine and its derivatives. Am Chem Soc 93:7062–7069
Cardellini E, Cinelli S, Gianfranceschi G, Onori G, Santucci A (2000) Differential scanning calorimetry of chromatin at different levels of condensation. Mol Biol Rep 27:175–180
Durham T, Snow E (2006) Metals and carcinogenesis. EXS 96:97–130
Ellen T, Kluz T, Harder M, Xiong J, Costa M (2009) Heterochromatinization as a potential mechanism of nickel induced carcinogenesis. Biochemistry 48:4626–4632
Favier I, Massou S, Teuma E, Philippot K, Chaudret B, Gómez M (2008) A new a specific mode of stabilization of metallic nanoparticles. Chem Commun (Camb) 28:3296–3298
Henson M, Chedrese J (2004) Endocrine disruption by cadmium a common environmental toxicant with paradoxical effects on reproduction. Exp Biol Medicine (Maywood) 229:383–392
Jokhadze T, Lezhava T (1994) Study of chromosomes structural changes induced by heavy metal salt at in vivo and in vitro aging. Genetika (Russ) 30:1630–1632
Kawanishi S, Oikawa S, Inoue S, Nishino K (2002) Distinct mechanisms of oxidative DNA damage induced by carcinogenic nickel subsulfide and nickel oxides. Environ Health Perspect 1:789–791
Lezhava T (2001) Chromosome and aging: genetic conception of aging. Biogerontology 2:253–260
Lezhava T (2006) Human chromosomes and aging. From 80–114 years. New York–Nova Biomedical, p 177
Lezhava T, Jokhadze T (2007) Activation of pericentromeric and telomeric heterochromatin in cultured lymphocytes from old individuals. Ann N Y Acad Sci 1100:387–399
Lezhava T, Jokhadze T, Monaselidze J (2008) Decondensation of chromosomes Oikawa heterochromatinization regionsby effect of heavy metals and bioregulators in cultured lymphocytes from old individuals. Proceedings of the 10th International Symposium of Metal Ions in Biology and Medicine. Bastia France May 19–22 Edited by Philippe Collery 10:451–457
Mazin A (1994) Enzymatic DNA methylation as an aging mechanism. Mol Biol Mosc 28:21–51
Mazin A (2009) Suicidal function of DNA methylation in age-related genome disintegration. Ageing Res Rev 8:314–327
Monaselidze J, Abuladze M, Asatiani N, Kiziria E, Barbakadze Sh, Majagaladze G, Iobadze L, Tabatadze I, Holman H-Y, Sapojnikova N (2006) Characterization of chromium-induced apoptosis in cultured mammalian cells. A different scanning calorimetry study. Thermochem Acta 441:8–15
Monaselidze J, Bregadze V, Barbakadze Sh, Majagaladze G, Khachidze D, Kiladze M, Kuchadze Z, Lezhava T, Jokhadze T (2008) Influence of metal ions of thermodynamic stability of leukemic DNA in vivo. Microcalorimetric investigation. Proceedings of the 10th International Symposium of Metal Ions in Biology and Medicine Bastia France, May 19–22. Edited by Philippe Collery 10: 451–457
Muramatsu S, Hanada H, Himeno K (1980) Effect of cadmium in biological tissues by atomic absorption induction in bone marrow cells and spermatogonia on mice. Radiobiological Equivalents. Chem Pollit Proc Advis Group Meet 61
Nemeth A, Langst G (2004) Chromatin higher order structure: opening up chromatin for transcription. Brief Funct Genomic Proteomic 2:334–343
Prokofieva-Belgovskaya A (1986) Chromatin regions of chromosomes. M Nauka, 431
Theophanides T, Anastassopoulou J (2002) Copper and carcinogenesis. Crit Rev Oncol Hematol 42:57–64
Turner B, Koehane A (1986) Cu2+-dependent changes in metaphases chromosomes structure assayed by antibody labelling and flow cytometry. Biochem Soc Trans 4:1166–1167
Uhlmann K (2001) Chromosome condensation: packaging the genome. Curr Biol 11:R384–R387
Valko M, Morris H, Cronin M (2005) Metals, toxicity and oxidative stress. Curr Med Chem 12:1161–1208
Valko M, Rhodes C, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 10:1–4
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Lezhava, T., Monaselidze, J., Jokhadze, T. et al. Remodeling of heterochromatin induced by heavy metals in extreme old age. AGE 33, 433–438 (2011). https://doi.org/10.1007/s11357-010-9185-1
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DOI: https://doi.org/10.1007/s11357-010-9185-1