Abstract
With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa.
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Acknowledgements
This study was supported by the research fund of the Japan Kampo Medicine Manufacturers Association and a grant from the High-Tech Research Center Establishment Project of the Ministry of Education, Culture, Sports, Science and Technology.
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Minami, M., Nishio, K., Ajioka, Y. et al. Identification of Curcuma plants and curcumin content level by DNA polymorphisms in the trnS-trnfM intergenic spacer in chloroplast DNA. J Nat Med 63, 75–79 (2009). https://doi.org/10.1007/s11418-008-0283-7
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DOI: https://doi.org/10.1007/s11418-008-0283-7