Skip to main content
SpringerLink
Log in

Enzymatic kinetic of cellulose hydrolysis

Inhibition by ethanol and cellobiose

  • Original Articles
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The ethanol effect on the Trichoderma reesei cellulases was studied to quantify and clarify this inhibition type. To determine inhibition parameters of crude cellulase and purified exoglucanase Cel7A, integrated Michaelis-Menten equations were used assuming the presence of two inhibitors: cellobiose as the reaction product and ethanol as a possible bioproduct of cellulose fermentation.

It was found that hydrolysis of cellulose by crude enzyme follows a model that considers noncompetitive inhibition by ethanol, whereas Cel7A is very slightly competitively inhibited. Crude cellulase is much more inhibited (K iul=K icl=151.9 mM) than exoglucanase Cel7A (K icl=1.6 × 1015 mM). Also, calculated inhibition constants showed that cellobiose inhibition is more potent than ethanol inhibition both for the crude enzyme as well as exoglucanase Cel7A.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pettersson, P. O., Eklund, R., and Zacchi, G. (2002), Appl. Biochem. Biotechnol. 98–100, 733–746.

    Article  Google Scholar 

  2. Sun, Y. and Cheng, J. (2004), Biotechnol. Prog. 20, 698–705.

    Article  CAS  Google Scholar 

  3. Eriksson, T., Karlsson, J., and Tjerneld, F. A. (2002), Appl. Biochem. Biotech. 101, 41–60.

    Article  CAS  Google Scholar 

  4. Rabinovich, M. L., Melnick, M. S., and Bolovova, A. V. (2002) Appl. Biochem. Microbiol. 38, 305–321.

    Article  CAS  Google Scholar 

  5. Ooshima, H., Ishitani, Y., and Harano, Y. (1985), Biotechnol. Bioeng. 27, 389–397.

    Article  CAS  Google Scholar 

  6. Holtzapple, M., Cognata, M., Shu, Y., and Hendrickson, C. (1990), Biotechnol. Bioeng. 36, 275–287.

    Article  CAS  Google Scholar 

  7. Kennedy, J. F. and Cabral, J. M. S. (1987), in Biotechnology, vol. 7a (Rehm, H. J. and Reed, G., eds.), pp. 253–391.

  8. Caldini, C., Bonomi, F., Pifferi, P. G., Lanzarini, G., and Galante, Y. M. (1994), Enzyme Microb. Technol. 16, 286–291.

    Article  CAS  Google Scholar 

  9. Takagi, M. (1984), Biotechnol. Bioeng. XXVI, 1506–1507.

    Article  Google Scholar 

  10. Wu, Z. and Lee, Y. Y. (1997), Biotechnol. Lett. 19, 977–979.

    Article  CAS  Google Scholar 

  11. Orsi, B. A. and Tipton, K. F. (1979), Methods Enzymol. 63, 159–183.

    CAS  Google Scholar 

  12. Duggleby, R. G. (1986), Biochem. J. 235, 613–615.

    CAS  Google Scholar 

  13. Howell, J. A. and Stuck, J. D. (1975), Biotechnol. Bioeng. 17, 873–893.

    Article  CAS  Google Scholar 

  14. Hsu, T.-A. and Tsao, G. T. (1979), Biotechnol. Bioeng. 21, 2235–2246.

    Article  CAS  Google Scholar 

  15. Hsu, T.-A., Gong, C.-S., and Tsao, G. T. (1980), Biotechnol. Bioeng. 12, 2305–2320.

    Article  Google Scholar 

  16. Pereira, A. N. (1987), PhD Thesis, Purdue University, West Lafayette, IN.

    Google Scholar 

  17. Nidetzky, B., Hayn, M., Macarron, R., and Steiner, W. (1993), Biotechnol. Lett. 15, 71–76.

    Article  CAS  Google Scholar 

  18. Bezerra, R. M. F. and Dias, A. A. (2004) Appl. Biochem. Biotechnol. 112, 173–184.

    Article  CAS  Google Scholar 

  19. Jones, E. and Weatherley, L. R. (2003), J. Chem. Technol. Biotechnol. 78, 194–198.

    Article  CAS  Google Scholar 

  20. Foster, R. J. and Niemann, C. (1955), J. Am. Chem. Soc. 77, 1886–1892.

    Article  CAS  Google Scholar 

  21. Philo, R. D. and Selwyn, M. J. (1973) Biochem. J. 135, 525–530.

    CAS  Google Scholar 

  22. Wood, T. M. and Bhat, K. M. (1988), Methods Enzymol. 160, 87–113.

    CAS  Google Scholar 

  23. Bezerra, R. M. F. (1999) J. Med. Biochem. 3, 9–16.

    CAS  Google Scholar 

  24. Hsu, T.-A. (1979), PhD Thesis, Purdue University, West Lafayette, IN.

    Google Scholar 

  25. Mannervik, B. (1982), Methods Enzymol. 87C, 370–391.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CETAV-Departamento de Engenharia Biológica e Ambiental, Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5001-911, Vila Real, Portugal

    Rui M. F. Bezerra & Albino A. Dias

Authors
  1. Rui M. F. Bezerra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Albino A. Dias
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rui M. F. Bezerra.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bezerra, R.M.F., Dias, A.A. Enzymatic kinetic of cellulose hydrolysis. Appl Biochem Biotechnol 126, 49–59 (2005). https://doi.org/10.1007/s12010-005-0005-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-005-0005-5

Index Entries

Search

Navigation