Abstract
In this study, different enzyme preparations available from Novozymes were assessed for their efficiency to hydrolyze lignocellulosic materials. The enzyme mixture was evaluated on a pretreated cellulose-rich material, and steam-exploded barley straw pretreated under different temperatures (190, 200, and 210°C, respectively) in order to produce fermentable sugars. Results show that xylanase supplementation improves initial cellulose hydrolysis effectiveness of water-insoluble solid fraction from all steam-exploded barley straw samples, regardless of the xylan content of substrate. The mixture constituted by cellulase: β-glucosidase: endoxylanase of the new kit for lignocellulose conversion at a ratio 10:1:5% ([v/w], enzyme [E]/substrate [S]) provides the highest increment of cellulose conversion in barley straw pretreated at 210°C, for 10 min.
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García-Aparicio, M.P., Ballesteros, M., Manzanares, P. et al. Xylanase contribution to the efficiency of cellulose enzymatic hydrolysis of barley straw. Appl Biochem Biotechnol 137, 353–365 (2007). https://doi.org/10.1007/s12010-007-9064-0
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DOI: https://doi.org/10.1007/s12010-007-9064-0