Abstract
Dehydrin gene transcript could be induced by heavy metals, and some dehydrins possess the ability to bind metals. However, the correlation between dehydrins and heavy-metal stress is unknown. In order to elucidate the contribution of dehydrins to heavy-metal stress tolerance in plants, we cloned two SK2-type dehydrin genes from heavy-metal hyperaccumulator Brassica juncea, and investigated their Cd/Zn tolerance in transgenic plants. Semi-quantitative RT-PCR analysis revealed that BjDHN2/BjDHN3 expressed in the leaves, stems and roots at a low level and were up-regulated by heavy metals. Antisense BjDHN3 Brassica juncea plants showed more electrolyte leakage and higher malondialdehyde production than the control plants when exposed to heavy metals, and the total amount of metals accumulated in the whole plant was reduced. Transgenic tobacco plants overexpressing BjDHN2/BjDHN3 showed lower electrolyte leakage and malondialdehyde production than the control plants when exposed to Cd/Zn. These results indicated that BjDHN2/BjDHN3 enhanced the tolerance for heavy metals by reducing lipid peroxidation and maintaining membrane stability in the plants.
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Acknowledgments
The authors would like to thank Prof. Alan Williams of the Graduate University of Chinese Academy of Sciences for his critical review of the English manuscript. The research was supported by the National High Technology Planning Program of China (Grant nos. 2006AA10Z407 and 2006AA06Z355), and China National Natural Sciences Foundation (Grant no. 30570146).
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Xu, J., Zhang, Y.X., Wei, W. et al. BjDHNs Confer Heavy-metal Tolerance in Plants. Mol Biotechnol 38, 91–98 (2008). https://doi.org/10.1007/s12033-007-9005-8
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DOI: https://doi.org/10.1007/s12033-007-9005-8