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Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma

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Abstract

Although cervical cancer is preventable with early detection, it remains the second most common malignancy among women. An understanding of how proteins change in their expression during a particular diseased state such as cervical cancer will contribute to an understanding of how the disease develops and progresses. Potentially, it may also lead to the ability to predict the occurrence of the disease. With this in mind, we aimed to identify differentially expressed proteins in the plasma of cervical cancer patients. Plasma from control, cervical intraepithelial neoplasia (CIN) grade 3 and squamous cell carcinoma (SCC) stage IV subjects was resolved by two-dimensional gel electrophoresis and the resulting proteome profiles compared. Differentially expressed protein spots were then identified by mass spectrometry. Eighteen proteins were found to be differentially expressed in the plasma of CIN 3 and SCC stage IV samples when compared with that of controls. Competitive ELISA further validated the expression of cytokeratin 19 and tetranectin. Functional analyses of these differentially expressed proteins will provide further insight into their potential role(s) in cervical cancer-specific monitoring and therapeutics.

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Abbreviations

2DE:

two-dimensional gel electrophoresis

ACHCA:

α-cyano-4-hydroxycinnamic acid

ANOVA:

analysis of variance

cELISA:

competitive ELISA

CIN:

cervical intraepithelial neoplasia

FIGO:

Federation of International Gynecologists and Obstetricians

HRP:

horseradish peroxidase

IEF:

isoelectric focusing

Ig:

immunoglobulin

MALDI-TOF/TOF MS:

matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry

nCLU:

nuclear form of clusterin

Pap:

Papanicolaou

SCC:

squamous cell carcinoma

sCLU:

secretory form of clusterin

SIL:

squamous intraepithelial lesion

TNF:

tumour necrosis factor

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Authors and Affiliations

  1. UKM Medical Molecular Biology Institute, Kuala Lumpur, Malaysia

    Mee Lee Looi

  2. Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

    Saiful Anuar Karsani

  3. Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

    Mariati Abdul Rahman

  4. Department of Obstetrics and Gynecology, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

    Ahmad Zailani Hatta Mohd Dali

  5. Department of Pathology, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

    Siti Aishah Md Ali

  6. Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

    Wan Zurinah Wan Ngah & Yasmin Anum Mohd Yusof

  7. University of Malaya Centre For Proteomics Research (UMCPR), Kuala Lumpur, Malaysia

    Saiful Anuar Karsani

Authors
  1. Mee Lee Looi
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  2. Saiful Anuar Karsani
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  3. Mariati Abdul Rahman
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  4. Ahmad Zailani Hatta Mohd Dali
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  5. Siti Aishah Md Ali
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  6. Wan Zurinah Wan Ngah
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  7. Yasmin Anum Mohd Yusof
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Correspondence to Yasmin Anum Mohd Yusof.

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Looi, M.L., Karsani, S.A., Rahman, M.A. et al. Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma. J Biosci 34, 917–925 (2009). https://doi.org/10.1007/s12038-009-0106-3

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  • DOI: https://doi.org/10.1007/s12038-009-0106-3

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