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From bacterial genomics to metagenomics: concept, tools and recent advances

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Abstract

In the last 20 years, the applications of genomics tools have completely transformed the field of microbial research. This has primarily happened due to revolution in sequencing technologies that have become available today. This review therefore, first describes the discoveries, upgradation and automation of sequencing techniques in a chronological order, followed by a brief discussion on microbial genomics. Some of the recently sequenced bacterial genomes are described to explain how complete genome data is now being used to derive interesting findings. Apart from the genomics of individual microbes, the study of unculturable microbiota from different environments is increasingly gaining importance. The second section is thus dedicated to the concept of metagenomics describing environmental DNA isolation, metagenomic library construction and screening methods to look for novel and potentially important genes, enzymes and biomolecules. It also deals with the pioneering studies in the area of metagenomics that are offering new insights into the previously unappreciated microbial world.

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Authors and Affiliations

  1. Department of Zoology, University of Delhi, Delhi, 110 007, India

    Pooja Sharma, Hansi Kumari, Mukesh Kumar, Mansi Verma, Kirti Kumari, Shweta Malhotra & Rup Lal

  2. Department of Plant and Molecular Biology, University of Delhi, South Campus, Benito Juarez Marg, Delhi, 110 021, India

    Jitendra Khurana

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  1. Pooja Sharma
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  2. Hansi Kumari
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  3. Mukesh Kumar
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  4. Mansi Verma
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  7. Jitendra Khurana
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  8. Rup Lal
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Correspondence to Rup Lal.

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The authors have contributed equally to the work

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Sharma, P., Kumari, H., Kumar, M. et al. From bacterial genomics to metagenomics: concept, tools and recent advances. Indian J Microbiol 48, 173–194 (2008). https://doi.org/10.1007/s12088-008-0031-4

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